Ⅰ型胶原—整合素α2β1系统对成骨细胞生物学特性的调控

为进一步探讨Ⅰ型胶原及其受体系统是否为成骨细胞功能活动所必需，使用Ⅰ型胶原和整合素α２β１的一抗阻断Ⅰ型胶原－整合素α２β１系统，以细胞计数法比较成骨细胞的增殖能力，流式细胞仪分析成骨细胞的凋亡情况，ＲＴ－ＰＣＲ技术研究Ⅰ型胶原、整合素α２β１及骨钙素的ｍＲＮＡ表达情况。结果发现阻断Ⅰ型胶原－整合素α２β１系统后，成骨细胞的增殖能力减弱，凋亡率增高，Ⅰ型胶原、整合素α２β１及骨钙素的ｍＲＮＡ表达减

The modulation of type I collagen and its receptor system on biological characteristics of osteoblasts]

This paper addresses the question whether type I collagen-receptor system is necessary for the functional activity of osteoblasts. Through blocking type I collagen-receptor system by type I collagen antibody or integrin alpha 2 beta 1 antibody, the proliferation of osteoblasts was studied by cell count, the apoptosis was studied by flow cytometry, and the mRNA expressions of type I collagen, integrin alpha 2 beta 1 and osteocalcin were also detected by RT-PCR techniques. When type I collagen-receptor system was blocked, osteoblasts showed high apoptosis rate, lower growth kinetics and weak mRNA expression of type I collagen, integrin alpha 2 beta 1 and osteocalcin; the blocking effect was reversible. It suggests that type I collagen-receptor system is necessary for osteoblastic phenotype. The biomaterials for bone tissue engineering should be constructed according to the extracellular matrix of osteoblasts, which supply normal extracellular environment for osteoblasts.