镉还原法测定一氧化氮稳定代谢产物NO2^—／NO3^—的影响因素研究

为建立更角的一氧化氮（ＮＯ）间接测定法，作者比较了镉屑、５ｍｍｏｌ／Ｌ及８０ｍｍｏｌ／Ｌ铜离了活化镉屑对镉还原法测定ＮＯ稳定代谢产物ＮＯ２＾－／ＮＯ３＾－的影响。结果显示：镉屑对ＮＯ３＾－的还原效率明显优于铜离子活化镉屑（Ｐ〈０．００１），还原率可达９６％，且在去蛋白细胞培养液中同样显示出较强的还原效率（Ｐ〈０．０１）。结果提示：镉屑在生物液体中具良好的抗干扰性能。本法简便易行，不需昂设备，准确度

The influential factors in using cadmium reduction method for measurement of the stable products of NO--nitrates and nitrites]

To establish an efficient assay method for detecting nitric oxide indirectly, we compared the effects of cadmium filing, 5 mmol/L and 80 mmol/L copperized cadmium filing on the measurement of nitrates and nitrites (the stable products of NO) using the cadmium reduction method. The results demonstrated that cadmium filing was the most efficient cadmium preparations (P < 0.001). It's mean reduction rate was 96%, and it showed stronger reduction effectiveness in deproteinization cell culture media. The results indicated that cadmium filing has strong anti-interference capacity in biological fluid. We recommend the cadmium filing reduction method because it is simple, practical, inexpensive, highly efficient and can be performed in ordinary laboratories.