Caveolin-3在白藜芦醇改善高脂诱导骨骼肌胰岛素抵抗中的作用

 【目的】在高脂诱导的胰岛素抵抗大鼠模型上，探讨微囊蛋白3（CAV-3）在白藜芦醇改善骨骼肌胰岛素抵抗中的作用及机制。【方法】实验大鼠分三组:正常饮食组（NORM），高脂饮食组（HFD），高脂饮食加白藜芦醇干预组（HFD+ RSV）。通过腹腔糖耐量试验，空腹血糖和空腹血清胰岛素值以及2-脱氧-3H] 葡萄糖的摄取，从整体上探讨白藜芦醇对胰岛素抵抗的保护作用。再通过免疫印迹检验微囊蛋白3(CAV-3)与葡萄糖转运蛋白4（GLUT4）在以上过程中的表达情况。【结果】HFD能显著诱导胰岛素抵抗，补充RSV能抑制高脂的诱导作用。离体骨骼肌实验证实，RSV的保护作用与其促进胰岛素诱导的葡萄糖摄取有关。免疫印迹证实RSV能够增加骨骼肌CAV-3蛋白表达，促进GLUT4向细胞膜的转运。【结论】白藜芦醇能改善高脂诱导的胰岛素抵抗，其保护作用与其上调CAV-3蛋白表达，促进GLUT4向细胞膜转运，改善骨骼肌葡萄糖的摄取有关。

Role of Caveolin-3 in Resveratrol Protection against High-fat Diet-induced Insulin Resistance in Skeletal Muscle

【Objective】 In the high-fat-induced insulin resistance rat model,the role of caveolin-3(CAV-3) in the effect of resveratrol(RSV) on insulin resistance in skeletal muscles was studied.【Methods】 Rats were divided into three groups: normal diet group(NORM),high-fat diet group(HFD),high-fat diet plus resveratrol group(HFD + RSV).The protective effect of resveratrol on insulin resistance were studied by measuring fasting glucose,fasting serum insulin,insulin sensitive index(ISI),intraperitoneal glucose tolerance test,and 2-deoxy- glucose uptake in vitro soleus muscles.Expression of caveolin-3(CAV-3) and glucose transporter 4(GLUT4) in the above processes were measured by Western blot analysis.【Results】 RSV has protective effect on high-fat diet-induced insulin resistance.RSV elevated insulin-stimulated glucose uptake in isolated soleus muscle in vitro by enhancing GLUT4 translocation to the plasma membrane rather than increasing GLUT4 protein expression.RSV increased CAV-3 protein expression,which contributed to GLUT4 translocation.【Conclusion】 The results showed that RSV ameliorates insulin resistance by increasing glucose uptake in skeletal muscles through a CAV-3-dependent pathway,and the mechanism was that RSV up regulates CAV-3 protein expression and enhances GLUT4 translocation to plasma membrane.