| 热解离试验合并ELISA检测微量血痕的MN血型 |
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| 引用本文： | 何小平,郭景元.热解离试验合并ELISA检测微量血痕的MN血型[J].中山大学学报(医学科学版),1989(2). |
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| 作者姓名： | 何小平 郭景元 |
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| 作者单位： | 中山医科大学法医系物证教研室，中山医科大学法医系物证教研室 研究生 |
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| 摘 要： | 用酶联免疫吸附测定法(ELISA)代替指示红细胞检测血痕热解离液中的微量血型抗体,通过酶促反应判定血痕的血型。并根据血痕抗N侧反应的强弱区别MN型血痕的抗N反应和M型血痕的“类N”反应。实验证明用ELISA检测溶液中微量MN血型抗体比用指示红细胞作凝集反应更为敏感。共检测血痕625例。九年以内的N型血痕、3个月以内的M型血痕、2个月以内的MN型血痕,检测的正确率为100％。
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| 关 键 词： | 热解离试验 酶联免疫吸附测定法 血痕 MN血型 酶标抗体 |
MN Typing in Micro-Bloodstains by Elution Test with Enzymes-Linked Immunosorbent Assay (ELISA) |
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| He Xiaoping Guo Jingyuan.MN Typing in Micro-Bloodstains by Elution Test with Enzymes-Linked Immunosorbent Assay (ELISA)[J].Journal of Sun Yatsen University(Medical Sciences),1989(2). |
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| Authors: | He Xiaoping Guo Jingyuan |
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| Institution: | Faculty of Forensic Medicine |
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| Abstract: | A method for detecting MN blood groups in bloodstains by elution test with ELISA is reported. Bloodstain elution fluid contains micro specific antibodies which were detected by ELISA. The existence of the micro specific antibodies was showed by enzyme-catalyzing reaction and MN blood groups were interpreted, We could distinguish N activity of MN blood group and N-like activity of M blood group according to their reaction intensity. Result shows that it is more sensitive to detect micro specific antibodies in fluid by ELISA than by indicating red blood cells, |
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| Keywords: | Bloodstain elution test MN blood group Enzyme-Linked immunosorbent assay (ELISA) Enryme-Iabled antibody |
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