正常人与囊性纤维化病人汗腺细胞膜单离子通道对Forskolin的反应

本文报道用片膜钳技术研究正常人及囊性纤维化病人(CF)的汗腺上皮细胞膜单个离子通道活动的特性,并比较分析了它们对Forskolin的反应。在400多个膜片上找出了至少七种或七个状态的离子通道的特性。观察到正常人和CF的部分汗腺上皮细胞(包括腺细胞与管细胞)膜离子通道对Forskolin有兴奋反应。发现腺细胞的兴奋反应率在正常人明显高于CF病人,差异非常显著。而管细胞的兴奋反应率,两组问无明显差异。以上结果提示,用Forskolin可协助鉴定CF的缺损,但宜选用腺细胞,不宜选用管细胞。

Single Ion Channel Responses of Sweat Gland Cell Membrane from Normal Individuals and Patients with Cystic Fibrosis to Forskolin

The ion channel properties of sweat gland epithelial cell membrane from normal individuals and patients with cystic fibrosis (CF) were investigated by patch clamp technique. More than 400 cells were recorded and at least 7 types of channels or channel states were observed. Effects of Forskolin on channels were found to be different between the CF and the normal subjects: The ratio of excitation induced by Forskolin in secretory gland cell membrane normal subjects was much higher than that from the CF. The difference had statistical significant (p< 0.005). But for the reabsorptive duct cells, there was no significant difference between the normal and the CF (p>0.5). There results indicate that Forskolin could be help in identifying the abnormality of CF defected gene. But gland cells instead of duct cells should be chosen.