甘西鼠尾草愈伤组织诱导、增殖及分化研究

目的 考察不同质量浓度激素组合对甘西鼠尾草愈伤组织诱导、增殖及分化的影响。方法 以甘西鼠尾草叶片为外植体，采用正交试验研究2，4-D、NAA、6-BA、KT对愈伤组织诱导及增殖的影响，并探讨适宜愈伤组织分化的培养条件。结果 以诱导率和相对生长速率为指标，6-BA和2，4-D均对甘西鼠尾草细胞脱分化和细胞增殖具有显著影响，愈伤组织诱导的最佳激素组合为2，4-D 0.5 mg/L＋NAA 1.0 mg/L＋6-BA 0.5 mg/L，增殖的最佳激素组合为2，4-D 0.5 mg/L＋6-BA 2.0 mg/L＋KT 0.1 mg/L，MS＋NAA 0.5 mg/L＋6-BA 1.0 mg/L的培养基有利于不定芽的分化，而1/2 MS＋IBA 1.0 mg/L的培养基则有利于不定根的分化。结论 本实验所建立的组织培养体系，适宜甘西鼠尾草的植株再生。

Induction, proliferation, and differentiation on callus culture of Salvia przewalskii

Objective To investigate the effects of different concentrations of hormonal combinations on induction, proliferation, and differentiation in Salvia przewalskii callus culture. Methods Using sterile leaves of S. przewalskii as explants, the different concentrations of 2, 4-D, NAA, 6-BA, and KT on induction, proliferation of callus were optimized by orthogonal test. Meanwhile the suitable hormonal combinations for differentiation of callus were studied. Results The results showed that taking the inductive rate and the relative growth rate as norms, 6-BA and 2, 4-D had the most significant effects on the growth of cell division in S. przewalskii; The best hormonal combination for induction in S. przewalskii callus was 2, 4-D 0.5 mg/L + NAA 1.0 mg/L + 6-BA 0.5 mg/L and the best hormonal combination for proliferation in S. przewalskii callus was 2, 4-D 0.5 mg/L + 6-BA 2.0 mg/L + KT 0.1 mg/L. The medium of MS + NAA 0.5 mg/L + 6-BA 1.0 mg/L was beneficial to the differentiation of adventitious buds, and 1/2 MS + IBA 1.0 mg/L was helpful for the differentiation of adventitious roots. Conclusion The system of organization culture which is established in this study is suitable for the regeneration of S. przewalskii.