水半夏组培快繁体系的建立

目的 建立水半夏的组培快繁体系，为快速、大量生产水半夏种苗提供基础。方法 利用不同植物激素配比的培养基，以水半夏块茎为外植体进行试验。结果 愈伤培养基MS＋NAA 0.2 mg/L＋KT 1.0 mg/L＋蔗糖3 %＋琼脂6 g/L可成功诱导水半夏愈伤组织；丛生芽培养基MS＋NAA 0.2 mg/L＋6-BA 2.0 mg/L＋蔗糖3 %＋琼脂6 g/L诱导不定芽效果较好，增殖倍数高；生根培养基1/2 MS＋NAA 0.2 mg/L＋IBA 0.4 mg/L＋KT 0.2 mg/L＋蔗糖3 %＋琼脂6 g/L＋AC 0.3 g/L，有利于水半夏不定根的快速形成，12～14周即可获得再生水半夏植株。培养基MS＋NAA 0.2 mg/L＋6-BA 1.5 mg/L＋蔗糖3 %＋琼脂6 g/L为诱导水半夏分化一次性成苗的最佳激素配比，5～6周可形成试管苗，10周后可用于炼苗。结论 建立的水半夏组培快繁体系为水半夏优良品种的快速繁殖奠定基础，且可应用于工厂化生产水半夏种苗。

Establishment of tissue culture and rapid propagation system of Typhonium flagelliforme

Objective To establish the rapid propagation system and tissue culture techniques of Typhonium flagelliforme for large-scale seedlings. Methods Using media with different hormones proportions, the tuber tissue was found to be a good explant for inducing asexual propagation system. Results The callus culture medium consisted of MS+NAA 0.2 mg/L+KT 1.0 mg/L+sucrose 3%+agar 6 g/L could generate callus successfully. Medium consisted of MS+NAA 0.2 mg/L+6-BA 2 mg/L+sucrose 3 % + agar 6 g/L was a superlative proportion of hormones concentration to generate adventitious buds efficiently, especially for higher proliferated multiples. The rooting medium consisted of 1/2 MS+NAA 0.2 mg/L+IBA 0.4 mg/L+KT 0.2 mg/L+sucrose 3 %+agar 6 g/L+AC 0.3 g/L was conducive to generate roots rapidly. The explants could be acclimatized after a period of 12 to 14 weeks. Experiments of one-step-seedling formation indicated that the one-step-seedling formation medium, containing MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+sucrose 3%+agar 6 g/L was the best proportion of hormone concentration, for 5 to 6 weeks, new plantlets could be developed, which will be acclimatized in 10 weeks. Conclusion The tissue culture techniques and rapid propagation system of T. flagelliforme could be used for large-scale seedlings and lay a foundation for its improved breeds.