制备液相色谱分离制备玉郎伞查尔酮类化合物

目的 对壮药玉郎伞查尔酮类化合物进行分离制备和结构鉴定。方法 利用制备液相色谱分离、制备查尔酮类化合物，经HPLC测定其质量分数、用UV，IR，MS，1D、2D-NMR，X射线单晶衍射等对其进行结构鉴定。结果 从玉郎伞中首次分离制备出两种查尔酮类化合物，其结构分别为cis-2, 6-二甲氧基查尔酮和cis-17-甲氧基-7-羟基-苯骈呋喃查尔酮。结论 该方法制备玉郎伞查尔酮类化合物质量分数很高，且简便、快速、可靠。

Isolation and preparation of chalcone compounds from tuber of Millettia pulchra var. laxior by pre-HPLC

Objective To isolate ande prepare chalcone compounds from the tuber of Millettia pulchra var. laxior (Yulansang, YLS) and identify their structures. Methods Pre-HPLC was used to prepare chalcone compounds and its purity was determined by HPLC. Several extensive spectral analyses, such as UV, IR, ESI-MS, X-ray monocrystal diffraction, and 1D, 2D-NMR were used to identify the chemical structures. Results Two chalcone compounds were isolated from YLS for the first time, their chemical structures were cis-2,6-dimethoxyl chalcone and cis-17-methoxyl-7-hydroxyl-benzofuran chalcone, respectively. Conclusion High purity compounds could be obtained from YLS by this methods.