巴曲酶注射液对改进光化学法致栓塞性脑梗死家兔模型的改善研究

目的 建立同时测定银黄颗粒中7种有机酸类成分（新绿原酸、绿原酸、隐绿原酸、咖啡酸和异绿原酸A、B、C）和4种黄酮类成分（黄芩苷、汉黄芩苷、黄芩素及汉黄芩素）的HPLC方法。方法 色谱柱为AkzoNobel Kromasil C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；分段变波长测定；柱温30 ℃；体积流量1.0 mL/min。结果 11种成分的线性关系均良好，精密度、稳定性、重复性的RSD均低于2%，加样回收率为97.35%～99.23%。结论 该方法简便、准确、灵敏，可用于银黄颗粒质量标准提高研究。

Improvement of Batroxobin Injection on photochemically-induced embolic cerebral infarction model in rabbits

Objective To study the improvement of Batroxobin Injection on photochemically-induced embolic cerebral infarction model in rabbits. Methods Rabbits were randomly divided into Sham operation, model, high-, and low-dose Batroxobin Injection groups (0.005, 0.015 BU/kg). The embolic cerebral infarction model was induced by photochemical method, then the effect of Batroxobin Injection was observed. Results When the cerebral infarction was caused by improvd photochemical method, the cerebral blood flow reduced in each group. The cerebral blood flow in high- and low-dose Batroxobin Injection groups reduced over 50% at 120 min compared with the Sham group. Even more the damaged nerve function and cerebral infarction were observed, which proved that the model was scientific and reliable. After Batroxobin Injection administration at a dose of 0.005 and 0.015 BU/kg, the cerebral blood flow increased remarkably, and the nerve function and cerebral infarction were also reduced. As well, the research demonstrated that the fibrinogen (FIB) decreased and the tissue-type plasminogen activator (t-PA) increased. More over, there was no remarkable hemorrhage side reaction. Conclusion A model of rabbit focal cerebral infarction with the improvement is established by photochemical method, which is successful and may be reliable in the evaluation of antithrombotic drug research. Meanwhile, Batroxobin Injection could improve the damaged nerve function and narrow the cerebral infarction by this model.