红芪总多糖对荷S180瘤小鼠的抑瘤作用及其机制的研究

目的 研究红芪总多糖（THPS）单用及与环磷酰胺（CTX）合用对荷瘤小鼠的抑瘤作用，并探讨其机制。方法 昆明种小鼠90只，随机分10只为正常对照组，其余80只小鼠接种S180瘤株后随机分为荷瘤对照组、CTX组、THPS高、中、低剂量 400、200、100 mg/(kg·d)] 组及各与CTX合用组共8组。正常对照组和荷瘤对照组给予生理盐水，其余各组给予相应剂量THPS及CTX治疗，观察肿瘤的体积变化。14 d后处死小鼠，观察瘤质量、抑瘤率；血球计数仪检测外周血白细胞、血小板、红细胞及血红蛋白；流式细胞仪检测CD3⁺ T细胞和NK细胞。结果 与荷瘤对照组相比，中剂量THPS明显抑制小鼠S180瘤质量增长（P＜0.01）及体积增加（P＜0.05），提高NK细胞水平（P＜0.05）。与CTX组相比，THPS与CTX合用后，各剂量组均能明显抑制CTX所致的白细胞数量下降（P＜0.01）；而只有中剂量的THPS与CTX合用能够降低肿瘤的质量（P＜0.05），并能明显抑制CTX所致的CD3⁺T细胞（P＜0.01）和NK细胞（P＜0.05）数量下降。结论 中剂量的THPS能抑制S180瘤生长，具有降低CTX免疫抑制和骨髓抑制的作用，其机制与提高T细胞和NK细胞介导的免疫应答有关。

Antitumor effect of total hedysarum polybotrys saccharide on mice with S180 and its mechanism

Objective To investigate the antitumor effects of total hedysarum polybotrys saccharide (THPS) alone and THPS combinated with cytoxan (CTX) on tumor-bearing mice and its mechanisms. Methods Ninety Kunming mice were divided into nine groups randomly. Except for normal group, other eight groups inoculated with S180 tumor cells consisted of control, CTX, THPS of high, moderate, and low doses, and THPS of high, moderate, and low doses combined with cytoxan groups. Normal and control groups were treated with normal saline while others were treated with CTX, THPS, and THPS combination with CTX. Tumor sizes were measured every other day. All mice were killed after treatment for 14 d. Tumor weight and inhibition rate were observed. CD3⁺T lymphocyte and NK cells were counted through the flow cytometer. Blood routine was tested by blood cell count instrument. Results Compared with the control group, the moderate dose of THPS showed significantly antitumor effect on S180 tumor weight (P<0.01) and size (P<0.05) and markedly increased NK cell amount (P<0.05). Compared with the CTX group, all dose groups of THPS combined with CTX obviously inhibited the decrease of white blood cell amount (P<0.01). Only the moderate dose of THPS combined with CTX significantly reduced S180 tumor weight (P<0.05) and inhibited the decreases of CD3⁺ T lymphocyte (P<0.01) and NK cell (P<0.05) amounts caused by CTX. Conclusion The moderate dose of THPS showed significant antitumor effect in S180-bearing mice and reduced immunosuppression and myelosuppression caused by CTX through improving immune response mediated by T cells and NK cells.