红缘拟层孔菌液体发酵培养基配方的研究

目的：研究不同培养基组成成分对红缘拟层孔菌菌丝产量和多糖量的影响，优选出的红缘拟层孔菌液体发酵培养基的最佳配方。方法：以菌丝体生物量和胞内多糖的量为指标，通过单因素试验确定液体培养基中最佳碳源、氮源及维生素B₁的用量。通过L₉(3⁴)正交试验确定红缘拟层孔菌的液体发酵培养基配方的最佳组合。结果：优选出的红缘拟层孔菌液体发酵培养基最佳配方为（g/100 mL）：大米粉5，酵母粉1，磷酸二氢钾0.1，硫酸镁0.15，维生素B₁0.04。结论：优选出红缘拟层孔菌的液体发酵培养基的最佳配方，为今后大规模液体发酵研究奠定了基础。

Study on culture medium composition for liquid fermentation of Fomitopsis pinicola

Objective: To study the optimal culture medium composition for the liquid fermentation of Fomitopsis pinicola. Methods: Taking the mycelial biomass and production of polysaccharide as detecting indices, the optimum submerged fermentation parameters for F. pinicola. were studied by single factor test and orthogonal test. Results: The optimal formula for liquid fermentation (g/100 mL) were: rice powder 5, yeast 1, KH₂PO₃ 0.1, MgSO₄ 0.15, and VB₁ 0.04. Conclusion: The optimal medium composition for the liquid fermentation of F. pinicola has been selected, which provides the scientific basis for the further research of F. pinicola.