灵芝双向固体发酵雷公藤后菌质化学成分变化初步研究

目的 对灵芝双向固体发酵雷公藤所得菌质化学成分的变化进行初步研究，针对性地分离并鉴定出发酵过程中有明显变化规律的化学成分。方法 采用高效液相色谱法对雷公藤药材及灵芝双向固体发酵雷公藤所得到的不同时间点灵雷菌质的醋酸乙酯提取物进行化学成分分析，采用溶剂法、柱色谱法、高效液相制备法、核磁共振等对HPLC色谱图中部分具有明显变化规律的化学成分进行提取分离、结构鉴定。结果 与雷公藤生药相比，发酵所得菌质的液相色谱图具有明显规律性的改变；针对性地分离并鉴定出4个化合物，分别为12-甲氧基雷酚萜（1）、雷酚萜（2）、邻苯二甲酸二异丁酯（DIBP，3）、邻苯二甲酸二丁酯（DBP，4）；其中菌质中化合物DIBP、DBP的量随发酵时间的延长而降低。结论 灵芝双向固体发酵雷公藤的过程中，菌质中DIBP、DBP等邻苯二甲酸酯类有毒化合物的量降低。

Chemical constituents changes in Tripterygium wilfordii after ganoderma bidirectional solid fermentation

Objective To preliminarily study the chemical constituents change of Tripterygium wilfordii after ganoderma bidirectional solid fermentation and to isolate and identify the significant variation chemical constituents in the fermentation process. Methods HPLC was used to analyze the chemical constituents in T. wilfordii and ethyl acetate extract of T. wilfordii after ganoderma bidirectional solid fermentation. Solvent method, column chromatography, and HPLC preparation method were adopted to extract compounds with significant variation and NMR was used to identify their compounds. Results Compared with the HPLC chromatogram of T. wilfordii crude drug, mycoplasma chromatogram changed regularly and obviously. Four compounds were separated and identified as 12-methoxy-triptonoterpene (1), triptonoterpene (2), diisobutyl-phthalate (DIBP, 3), and dibutyl-phthalate (DBP, 4). The contents of DBP and DIBP were reduced with the extension of fermentation time. Conclusion During the process of fermenting, the contents of toxic chemicals such as DBP and DIBP are reduced. Because of complex chemical constituents changes, T. wilfordii after ganoderma bidirectional solid fermentation has lower toxicity than that in crude drug.