| rhBMP-2对再生障碍性贫血小鼠骨髓造血恢复的促进作用 |
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| 引用本文： | 高巧艳,王靖,邢万里,戴凯,刘昌胜.rhBMP-2对再生障碍性贫血小鼠骨髓造血恢复的促进作用[J].医学教育探索,2016(1):42-47. |
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| 作者姓名： | 高巧艳 王靖 邢万里 戴凯 刘昌胜 |
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| 作者单位： | 华东理工大学材料科学与工程学院, 教育部医用生物材料工程研究中心, 上海 200237,华东理工大学材料科学与工程学院, 教育部医用生物材料工程研究中心, 上海 200237,华东理工大学材料科学与工程学院, 教育部医用生物材料工程研究中心, 上海 200237,华东理工大学材料科学与工程学院, 教育部医用生物材料工程研究中心, 上海 200237,华东理工大学材料科学与工程学院, 教育部医用生物材料工程研究中心, 上海 200237 |
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| 基金项目： | 纳米研究重大科学研究计划(2012CB933600) |
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| 摘 要： | 探究了人重组骨形态发生蛋白-2(rhBMP-2)对再障小鼠的治疗作用。采用5-FU联合白消安建立小鼠再障模型,通过干预rhBMP-2进行治疗。考察了各实验组小鼠白细胞数、骨髓单核细胞数、体重、存活率、脾系数、粒系-巨系细胞集落(CFU-GM)数以及骨髓单核细胞中CD34+细胞比例,进行股骨、脾脏HE切片分析。结果表明,相较于再障对照组,rhBMP-2治疗组小鼠的白细胞数、存活率、CFU-GM集落数及骨髓单核细胞数显著提高,并且骨髓单核细胞中的CD34+细胞含量也提高,显著改善脾脏功能和缓解骨髓抑制,证实rhBMP-2能够促进再障小鼠造血损伤的修复。
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| 关 键 词： | 重组人骨形态发生蛋白-2 再生障碍性贫血 造血损伤修复 |
| 收稿时间： | 2015/5/14 0:00:00 |
Promoting Effects of rhBMP-2 on Aplastic Anemia Mice Bone Marrow Hematopoietic Recovery |
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| GAO Qiao-yan,WANG Jing,XING Wan-li,DAI Kai and LIU Chang-sheng.Promoting Effects of rhBMP-2 on Aplastic Anemia Mice Bone Marrow Hematopoietic Recovery[J].Researches in Medical Education,2016(1):42-47. |
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| Authors: | GAO Qiao-yan WANG Jing XING Wan-li DAI Kai and LIU Chang-sheng |
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| Institution: | Engineering Research Center of Medical Biomaterials, Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China,Engineering Research Center of Medical Biomaterials, Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China,Engineering Research Center of Medical Biomaterials, Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China,Engineering Research Center of Medical Biomaterials, Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China and Engineering Research Center of Medical Biomaterials, Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China |
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| Abstract: | Aiming to explore the promoting effects of rhBMP-2 on aplastic anemia mice bone marrow hematopoietic recovery,we built the hematopoietic injury model by 5-FU and busulfan with the interventional treatment of rhBMP-2.We analysed the number of mice peripheral white blood cells,bone marrow mononuclear cells,BM CD34+ cells,weight index,survival rate,spleen coefficient,CFU-GM colony as well as the HE sections of femur and spleen tissue.The results showed that rhBMP-2 can improve the index of peripheral white blood cell amount,mice survival rates,CFU-GM colony number,the bone marrow mononuclear cells number and CD34+ cells ratio;rhBMP-2 can also improve the function of the spleen and reduce bone marrow suppression compared with the AA control group.All results above confirmed that rhBMP-2 can effectively promote the restoration of hematopoietic injury in aplastic anemia mice. |
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| Keywords: | human bone morphogenetic protein-2(rhBMP-2) aplastic anemia hematopoietic damage repair |
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