首页 | 本学科首页   官方微博 | 高级检索  
检索        

灯心草及野灯心草中菲类成分的LC-ESI-MS快速识别及鉴定
引用本文:赵丹丹,李贵云,王小红,王 杨,林宏英,黄建梅.灯心草及野灯心草中菲类成分的LC-ESI-MS快速识别及鉴定[J].医学教育探索,2013,44(12):1539-1545.
作者姓名:赵丹丹  李贵云  王小红  王 杨  林宏英  黄建梅
作者单位:北京中医药大学中药学院,北京 100102
基金项目:国家自然科学基金资助项目(81173541);北京中医药大学科研创新团队项目(2011-CXTD-14)
摘    要:目的 快速识别及鉴定灯心草及野灯心草中的菲类成分。方法 运用高效液相色谱-电喷雾质谱(HPLC-ESI-MS)联用技术研究菲类成分的裂解规律,并分别对灯心草和野灯心草甲醇提取液中的菲类成分进行快速识别和鉴定。结果 在负离子模式下,菲类成分容易丢失2H的碎片,推测可能是乙烯基与苯环环合而失去2个氢;也易发生重排丢失羰基,呈现出一定的裂解规律。根据菲类成分的裂解规律,从灯心草和野灯心草甲醇提取物中快速筛选了21个菲类成分,其中6个成分经与对照品比对而准确鉴定;另外结合质谱裂解信息及极性差异,推测出其中5个成分的结构。结论 HPLC-ESI-MS技术既可以从中药复杂体系中快速识别菲类成分并根据裂解规律初步确定菲类成分结构,也是实现中药中目标天然产物定向分离的有效手段。

关 键 词:灯心草  野灯心草  菲类  高效液相色谱-电喷雾质谱  质谱裂解

Rapid recognition and identification of phenanthrenes in Juncus effusus and Juncus setchuensis by LC-ESI-MS
ZHAO Dan-dan,LI Gui-yun,WANG Xiao-hong,WANG Yang,LIN Hong-ying,HUANG Jian-mei.Rapid recognition and identification of phenanthrenes in Juncus effusus and Juncus setchuensis by LC-ESI-MS[J].Researches in Medical Education,2013,44(12):1539-1545.
Authors:ZHAO Dan-dan  LI Gui-yun  WANG Xiao-hong  WANG Yang  LIN Hong-ying  HUANG Jian-mei
Institution:School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
Abstract:Objective To rapidly recognize and identify phenanthrenes in Juncus effusus and J. setchuensis. Methods HPLC-ESI- MS method was used to investigate the ESI-MS characteristics of those phenanthrenes, to screen, and to identify phenanthrenes in J. effusus and J. setchuensis. Results Under negative mode, phenanthrenes were inclined to loss two hydrogens, which presumably caused by the cyclization of vinyl and aromatic ring; Furthermore, the loss of C=O could be also observed. Fragmentation pathway of phenanthrenes in ESI as a negative ion mode was summarized. Twenty-one phenanthrenes were rapidly detected from the extracts of J. effusus and J. setchuensis. Among them, six compounds were accurately identified by comparison with reference substances; The structures of five compounds were inferred according to their MS spectrum and polarity. Conclusion HPLC-ESI-MS provides not only a new technique for the rapid identification of phenanthrenes from complex matrix, but also an effective method for the target separation of constituents in Chinese materia medica.
Keywords:Juncus effuses L    Juncus setchuensis Buchen    phenanthrenes  HPLC-ESI-MS  MS fragmentation
点击此处可从《医学教育探索》浏览原始摘要信息
点击此处可从《医学教育探索》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号