| *研究快报*玉米秸秆水解液的黑曲霉发酵生产高浓度柠檬酸 |
| |
| 引用本文： | 孟佼,周平平,张建,鲍杰.*研究快报*玉米秸秆水解液的黑曲霉发酵生产高浓度柠檬酸[J].医学教育探索,2014(6):681-683. |
| |
| 作者姓名： | 孟佼 周平平 张建 鲍杰 |
| |
| 作者单位： | 华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237 |
| |
| 基金项目： | 国家“973”计划（2011CB707406）；国家“863”项目(2012AA022301, 2014AA021901)；国家自然科学基金(21306048, 31300070)；中央高校基本科研业务费专项资金(WF1214025, 1314036) |
| |
| 摘 要： | 柠檬酸是在饮料、制药、化妆品等领域有广泛应用的重要有机酸。利用玉米秸秆等木质纤维素原料生产柠檬酸可以显著降低其生产成本。目前，木质纤维素柠檬酸发酵的技术指标偏低，不具备工业应用价值。本文以黑曲霉Aspergillus niger SIIM M288为发酵菌株，以干法稀酸预处理和生物脱毒后获得的玉米秸秆酶水解液为原料，在优化条件下柠檬酸发酵质量浓度达到97.45 g/L，相对于葡萄糖的得率达到了87.27%，是迄今为止所有报道的最高指标，已经接近以淀粉为原料的柠檬酸发酵指标。这一结果为以木质纤维素为原料生产柠檬酸的技术产业化提供了重要的依据。
|
| 关 键 词： | 柠檬酸 黑曲霉 玉米秸秆 木质纤维素 |
| 修稿时间： | 2014/9/14 0:00:00 |
High Titer Citric Acid Fermentation from Corn Stover Hydrolysate by Aspergillus niger |
| |
| MENG Jiao,ZHOU Ping-ping,ZHANG Jian and BAO Jie.High Titer Citric Acid Fermentation from Corn Stover Hydrolysate by Aspergillus niger[J].Researches in Medical Education,2014(6):681-683. |
| |
| Authors: | MENG Jiao ZHOU Ping-ping ZHANG Jian and BAO Jie |
| |
| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
| |
| Abstract: | Citric acid is one of the most versatile industrial organic acids in beverages, pharmaceutical, cosmetics, agriculture and biomedicine. Citric acid production using corn stover biomass as feedstock could provide an important way for cost reduction. The technical level of the reported citric acid production from lignocellulose biomass is far from the practical industry application. In this study, Aspergillus niger SIIM M288 was used to produce high titer citric acid using the corn stover hydrolysate as the substrate. Under the optimized condition, the citric acid titer and yield were achieved at 97.45 g/L and 87.27%, respectively, by Aspergillus niger SIIM M288 using corn stover hydrolysate. The results reached the highest level up to now. The results provided a possibility for economically citric acid production using the alternative lignocellulose feedstock. |
| |
| Keywords: | citric acid Aspergillus niger corn stover lignocellulosergillus niger |
|
| 点击此处可从《医学教育探索》浏览原始摘要信息 |
| 点击此处可从《医学教育探索》下载免费的PDF全文 |
|
