溪黄草离体培养和快速繁殖

目的：探讨溪黄草Isodon serra (Maxim).Kudo离培养的过程，为优良品种的快速繁殖奠定基础。方法：以溪贡草无菌苗的带节茎为外植体，采用MT基体培养基，附加不同植物激素进行试验。结果：培养基中附加激素BA有利于芽的发生；适宜的BA浓度为1mg/L，出芽率高达100％，且出芽数量多；基本培养基以完全的MT成分为好；生根据培养选择MT＋0．2mg/L NAA，生根率为100％，试管苗移栽，成活率为90％，结论，通过溪黄草离体培养的研究，获得了较高的植株再生频率，建立了有效的组织培养再生体系。

In vitro culture and rapid propagation of Isodo nserra

Object To study the in vitro culture of Isodon serra (Maxim ) Kudo, so as to lay a foundation for the rapid propagation of its improved breeds Methods The explants used for culture were stem segments with node from I serra The media were MT basal media containing different plant hormones Results The media containing BA were effective to induce shoot formation Optimal BA concentration for shoot formation and growth was 1 mg/L MT basal medium was better than 1/2 MT The optimal rooting medium for regenerated shoots was MT basal medium supplemented with 0 2 mg/L NAA, a root induction rate of 100% was achieved The survival rate of plantlet was 90% after transplanting under suitable condition Conclusion An effective plant regeneration system has been established for I serra on the basis of factors affecting the culture in vitro