植物生长调节剂对多花黄精芽体外发生过程中性状的影响

目的 研究不同植物生长调节剂组合对多花黄精芽体外发生过程中每块外植体上的平均芽数、叶片发生频率、根的自然发生率及生根外植体上的平均根数、根茎发生率等性状的影响；探索通过组织培养快速繁殖该植物的最佳途径。方法 多花黄精的不定芽切块在MS BA1．0mg／L 2，4-D0．5mg／L的培养基上可被诱导产生颗粒状愈伤组织，将该愈伤组织继代于不同植物生长调节剂组合的MS培养基上，2个月后观察并统计不同植物生长调节剂组合对各性状的影响。结果 TDZ1．5mg／L 2，4-D1．0mg／L对芽增值最有利，平均每块外植体上可长出8．6个芽；BAl．0～2．0mg／L NAA1．0～2．0mg／L促进叶片形成，95％以上的外植体长出形态正常的叶片；在BA2．0mg／L NAA1．0mg／L，的培养基上，根的自然发生率最高，达到51．9％，但不及单独生长素如NAA、IAA、TBA或2，4-D等以0．5～1．0mg／L，对根的诱导作用，后者可使90％以上的再生芽生根，且根生长繁茂；BA2．0mg／L 2，4-D1．0～2．0mg/L组合最适合根茎生长，直径大于0．5cm的根状茎超过100％。结论 多花黄精的体外快速繁殖可通过如下途径实现：TDZ1．5mg/L 2，4-D1．0mg/L诱导不定芽扩增，BA1．0～2．0mg／L NAA1．0～2．0mg/L诱导健康叶片的发育，1／2MS NAA，IAA，TBA或2，4-D0．5～1．0mg/L诱导再生芽生根；BA2．0mg/L 2，4-D1．0～2．0mg/L用于以繁殖药用器官为目的的根茎的生长。

Effect of plant growth regulators on several characteristics during in vitro bud regeneration of Polygonatum cyrtonema

Object To study the effects of plant growth regu la tors (PGR) with various combinations on several characteristics including number s o f per explant, production frequencies of leaves, abiogenesis of root and rhizome during in vitro bud regeneration process of Polygonatum cyrtonema Hua so as to search for the optimum in vitro regenera tion process for P. cyrtonema. Methods Granul ar calli were induced on MS+BA 1 0 mg/L+ 2, 4-D 0.5 mg/L by bud explant cuttings of P. cyrtonema. The ca lli were inoculated into MS media with various combined PGR. The characte ristics described above were observed and the effects of various combined PGR we ne obtained by statistics in two months R esults Among various combined PGR, TDZ 1.5 mg/L+2, 4-D 1.0 mg/L showed the best effect for the proliferation of buds, BA 1.0-2.0 mg/L+NAA 1 .0-2 .0 mg/L for improving of leave development, BA 2.0 mg/L+NAA 1.0 mg/L for root pr oduction and BA 2.0 mg/L+2, 4-D 1.0-2.0 mg/L for rhizome growth. The number of buds per explant was up to 8.6 in average, and the production rates of leaves, r oot, and rhizome over 0.5 cm in diameter were up to 95%, 51.9% and exceeding 100 %, respectively However, the medium containing only auxin such as NAA, IAA, IB A or 2, 4-D with 0.5-1.0 mg/L is much better for root induction of new buds tha n the original media with BA 2.0 mg/L+NAA 1.0 mg/L on which the explants can nat urally root. Conclusion The in vitro regener ation process of P. cyrtonema can be achieved by the following s teps: Firstly, proliferation of buds can be realized on medium with TDZ 1.5 mg/L +2, 4-D 1.0 mg/L. Then, normal leaves can be developed after the new buds have been transferred to medium with BA 1.0-2.0 mg/L+NAA 1.0-2.0 mg/L. Finally, vigor ous roots can be formed from regenerated buds on 1/2 MS medium with+ NAA, IAA, I BA or 2, 4-D 0.5-1.0 mg/L. Medium with BA 2.0 mg/L+2, 4-D 1.0-2.0 mg/L can be specially applied to improving the growth of rhizomes, the medicinal part of P. cyrtonema