survivin反义寡核苷酸诱导胃癌细胞凋亡及对多西他赛的增敏作用

目的探讨生存素（survivin）反义寡核苷酸（antisense oligonucleotide,ASODN）对人胃癌细胞株SGC7901的凋亡诱导、对多西他赛的化疗增敏作用。方法设计合成特异性靶向survivin ASODN。将胃癌细胞株分为空白对照组（Sham组）、单纯脂质体对照组（Lip组）、正义寡核苷酸转染对照组（Lip-SODN组）、ASODN转染组（Lip-ASODN组）。转染48 h后,Western blot法检测各组细胞survivin表达情况,流式细胞仪检测各组细胞凋亡率,MTT法检测转染细胞对多西他赛的敏感性。结果脂质体介导survivin ASODN转染后的胃癌细胞survivin蛋白表达明显下降,细胞凋亡率明显高于各对照组（P〈0.05）,对多西他赛的IC50值明显低于各对照组（P〈0.05）,细胞生长抑制率明显高于各对照组（P〈0.05）。结论survivin ASODN转染胃癌细胞能下调survivin蛋白表达,诱导胃癌细胞凋亡,抑制细胞增殖,增强多西他赛化疗敏感性。

Effects of survivin antisense oligonucleotide on inducing apoptosis and chemosensitivity to docetaxel of human gastric cancer cells

Objective To investigate the effects of survivin antisense oligonucleotide （ASODN） on the apoptosis and chemosensitivity to docetaxel of the human gastric carcinoma cell line SGC7901. Methods ASODN targeting survivin was designed and constructed. The cultured gastric carcinoma cells were divided into the sham group, lipofectin group, sense ollgonucleotide （SODN） group and antisense oligonucleotide （ASODN） group. After transfeetion for 48 h, the expression level of survivin in each group was detected by Western blot ; the apoptotic rate （AR） was examined by flow cytometry; and the sensitivity of SGC7901 cells to docetaxel was determined by MTT. Results The expression of survivin in SGC7901 cells was downregulated after transfection with survivin ASODN; The AR of ASODN group was significantly higher than that of the other groups （ P 〈 0.05 ） ; IC50 of the transfected cells to docetaxel had a significant difference as compared to other groups （ P 〈 0.05 ）, and the inhibitory rate （IR） of ASODN group was significantly higher than that of other groups （ P 〈 0.05）. Conclusion The ASODN transfection of gastric carcinoma cells can downregulate the survivin expression, induce cell apoptosis, inhibit cell proliferation and enhance the cell sensitivity to docetaxel, which may help to reverse drug resistance.