Livin和PCNA在口腔鳞癌浸润前沿中的表达和临床意义

目的检测凋亡抑制蛋白Livin和增殖细胞核抗原（PCNA）在口腔鳞癌浸润前沿和非前沿中的表达,探讨其在口腔鳞癌发生发展过程中的意义及两者相关性。方法对48例口腔鳞癌浸润前沿和非前沿部分分别进行病理分级,应用免疫组化Max-VisionTM快捷二步法检测Livin和PCNA在口腔鳞癌浸润前沿及非前沿部分的表达情况,并进行统计学分析。结果 48例口腔鳞癌浸润前沿和非前沿病理分级情况有显著性差异（P〈0.01）;Livin和PCNA在浸润前沿中的表达强度明显高于非前沿部分（P〈0.01）;Livin表达阳性的口腔鳞癌组织PCNA标记指数（LI）高于Livin表达阴性者（P〈0.01）,Livin的表达与PCNA呈正相关（rs=0.398,P=0.016）。结论口腔鳞癌浸润前沿肿瘤细胞凋亡抑制现象和增殖活性明显高于非前沿部分。浸润前沿中Livin和PCNA的高表达可以反映肿瘤的高恶性度和高复发转移倾向。

Expression and the clinical significance of livin and PCNA at the invasive tumor front of oral squamous cell carcinoma

Objective To detect the expression of apoptosis inhibitor livin and proliferating cell nuclear antigen（PCNA） at the invasive tumor front of oral squamous cell carcinoma,and investigate the clinical significance of livin and PCNA in the pathogenesis and progression of oral squamous cell carcinoma and their correlations.Method Forty-eight samples of oral squamous cell carcinoma were graded by invasive front grading（IFG） into invasive tumor front（ITF） and non-invasive tumor front（non-ITF）in pathology.The expression of livin and PCNA in both ITF and non-ITF was evaluated by two-step method of the rapid immunohistochemical staining（Max-VisionTM） and the results were analyzed statistically.Result There were significant differences in the ITF and non-ITF of oral squamous cell carcinoma as for the grading（P0.01）.The expression of livin and PCNA at the ITF was stronger than that of the non-ITF（P0.01）.The labeling index（LI） of PCNA in the positive group of expression of livin was higher than that in the negative group（P0.01）.The expression of livin was positively correlated with PCNA（rs=0.398,P=0.016）.Conclusion The apoptosis inhibition and proliferation of the tumor cells at the ITF in oral squamous cell carcinoma were more significant than at the non-ITF.The high expression levels of livin and PCNA at the ITF may indicate the trend of high malignancy,recurrence and metastasis.