| 人乳铁蛋白基因的克隆及基因序列多态性分析 |
| |
| 引用本文： | 杨光,庞庆丰,吴长毅,曾因明.人乳铁蛋白基因的克隆及基因序列多态性分析[J].徐州医学院学报,2005,25(5):390-392. |
| |
| 作者姓名： | 杨光 庞庆丰 吴长毅 曾因明 |
| |
| 作者单位： | 1. 徐州医学院江苏省麻醉学重点实验室,江苏,徐州,221002
2. 徐州医学院病理生理学教研室,江苏,徐州,221002 |
| |
| 基金项目： | 江苏省教育厅开放研究资助项目（KJSO4002） |
| |
| 摘 要： | 目的克隆人乳铁蛋白基因并进行基因序列分析.方法从人乳腺组织中提取出的总RNA进行逆转录,生成cDNA,以其为模板进行PCR扩增人乳铁蛋白cDNA.PCR产物纯化后连接到pGEM-T easy载体上并转化到DH5α菌中,然后对其进行DNA序列测定.结果本实验获得的基因序列与发表在GeneBank的hLF序列比较其同源性达99%,长度相符,为2149bp.与其他地区或国家相比较,有4～9个碱基有所不同.结论乳铁蛋白存在着基因序列多态性.
|
| 关 键 词： | 人乳铁蛋白 RT-PCR DNA序列分析 |
| 文章编号： | 1000-2065（2005）05-0390-03 |
| 收稿时间： | 07 11 2005 12:00AM |
| 修稿时间： | 2005-07-112005-09-08 |
Cloning of human lactoferrin gene and analysis of the polymorphism in its sequence |
| |
| Yang Guang;Pang QingFeng;Wu ChangYi;Ceng YinMing.Cloning of human lactoferrin gene and analysis of the polymorphism in its sequence[J].Acta Academiae Medicinae Xuzhou,2005,25(5):390-392. |
| |
| Authors: | Yang Guang;Pang QingFeng;Wu ChangYi;Ceng YinMing |
| |
| Abstract: | Objective To clone the gene of human lactoferrin and analyze its sequence.?Methods The total RNA extracted from normal human breast tissue was processed by RT-PCR to produce hLF cDNA.The product,after being purified,was connected to pGEMT easy vector.The conjugate was transferred into B.coli cell line,and the positive clones were screened for sequence analysis.?Results Comparison of the sequence of the hLF cDNA obtained with the material published in the genebank showed: there was a 99% homology,the consistant length was 2?149 bp,but 4-9 bp were found to be different from what were reported by other workers.?Conclusion hLF cDNA has polymorphism in sequence.Its cloning will contribute to the research work on human lactoferrin. |
| |
| Keywords: | human lactoferrin RT-PCR DNA sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
