骨髓间充质干细胞修复硬脊膜缺损的应用研究

目的探讨骨髓间充质干细胞（BMSC）修复兔破损硬脊膜的可行性和有效性。方法穿刺抽取日本大耳白兔髂后上棘骨髓，分离BMSC，采用贴壁筛选法对分离的BMSC进行纯化，接种于人工硬脊膜上。16只日本大耳白兔，随机分成A组、B组，每组8只，制作L4椎板切除硬脊膜破损模型，A组人工硬脊膜敷贴裂口处，B组骨髓间充质干细复合人工硬脊膜敷贴裂口处。术后30天处死动物，取修复区域的组织行大体观察和病理学观察，并对成纤维细胞密度进行分析。结果B组成纤维细胞与胶原纤维丰富，细胞密度高于A组（P〈0．05）。结论骨髓间充质干细胞可以促进硬脊膜修复。

Application of bone marrow mesenchymal stem cells to repair defects in spinal dura mater

Objective To explore the feasibility and effectiveness of bone marrow mesenchymal stem cells （ BMSCs） in repair of defects in rabbit dura mater. Methods The bone marrow was extracted from the ilium of rabbits. Then, BMSCs were isolated and purified through by density gradient centrifugation and adherence screening method before seeded onto artificial spinal dura mater. Sixteen rabbits were randomly divided into group A and group B, with eight in each group. An epidural damage model was made by removal of the L4 vertebral plate. Then, artificial spinal dura mater was padded into the gap in group A, while artificial spinal dura mater combined with bone marrow mesenchymal stem cells was padded into the gap in group B. The animals were sacrificed 30 days after operation and the tissue within the repaired area was collected for gross pathological and histological observation. Cell density was also calculated. Results Large amounts of fibroblasts and collagen fibers were observed in group B. Cell density in group B was higher than that of group A （ P 〈 0.05 ）. Conclusion Bone marrow mcscnchymal stem cells can promote the restoration of the dura mater.