Calbindin-D-28k对MPTP致小鼠黑质凋亡相关蛋白Bax表达的影响

目的探讨在1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）致小鼠黑质多巴胺（DA）能细胞发生凋亡时,calb ind in-D-28k（CB）抗细胞凋亡的作用机制。方法MPTP连续5天腹腔注射构建中脑黑质DA能细胞损伤的小鼠模型,模型鼠脑黑质致密部立体定位注射携带CB基因的高滴度慢病毒颗粒（CB-H IV-Ⅰ）,W estern b lotting方法检测CB的在体过表达以及鼠脑黑质部位凋亡相关蛋白Bax的表达变化。结果与空白对照组（control）和黑质定位注射空病毒颗粒组（H IV-Ⅰ）相比,注射CB-H IV-Ⅰ的实验组的黑质中CB的表达量显著升高（P〈0.05）,而Bax的表达量明显降低（P〈0.05）。结论病毒颗粒CB-H IV-Ⅰ携带的CB基因在黑质细胞中获得了高效的表达;凋亡相关蛋白Bax可能参与了CB对黑质DA能神经细胞的保护作用。

Effects of calbindin-D-28k on the expression of apoptosis-associated protein Bax in substantia nigra of mice with Parkinson's disease induced by MPTP

Objective To investigate the anti - apoptotic mechanism of calbindin - D - 28k （CB） in the MPTP - induced apoptosis of dopaminergic （DA） neurons in midbrain substantia nigra in mice. Methods MPTP was injected into the peritoneal cavity in 5 consecutive days to establish an injury model of DA neurons in the midbrain substantia nigra in mice, and high titer lentivirus particles carrying CB （ CB - HIV - Ⅰ ） were injected into the midbrain substantia nigra by stereotaxic apparatus. Western blotting was used to analyze the expression levels of CB and apoptosis - related protein Bax in substantia nigra, respectively. Results The expression level of CB increased （ P 〈 0.05）, while that of Bax decreased in CB - HIV - Ⅰ groups compared with control groups and HIV - Ⅰ groups （P 〈 0.05）. Conclusion The lentivirus particles of CB - HIV - Ⅰ were highly expressed in substantia nigra cells ; apoptosis - related protein Bax might participate in the protective effect of CB on DA neurons in substantia nigra.