骨髓基质细胞在气电纺丝蛋白纤维支架上早期粘附的研究

目的检测大鼠骨髓基质细胞在气电纺蚕丝蛋白纳米纤维支架上的早期粘附状况,研究其基本生物学性能.方法将具有良好生物性能的蚕丝蛋白通过气流高压静电纺丝工艺制成纳米纤维,并且通过激光共聚焦显微镜对骨髓基质细胞在气电纺蚕丝蛋白纳米纤维支架表面的早期粘附进行观察.结果骨髓基质细胞对气电纺蚕丝蛋白无纺织物表现出良好的亲和性,细胞在材料表面4h即形成了稳定的粘附,并有部分细胞开始在材料表面铺展,铺展细胞数与粘附细胞数的比率为75.9%,而对照组仅为49.63%.结论气电纺蚕丝蛋白纳米纤维支架具有良好的亲和性,提示其作为骨组织工程材料的可能性.

Early Adhesion of Bone Marrow Sroma Cells on Air/jet Electrospinning Silk Fibroin Nanofiber Scaffolds

Objective To study the biocompatibility of air/jet electrospinning silk fibroin nanofiber scaffolds by detecting the early adhesion of rat bone marrow stroma cells on the scaffolds.Methods In this study,the silk fibroin nanofiber was produced by air/jet Electrospinning,and the early adhesion of bone marrow stroma cells on the nanofiber scaffolds was observed by laser scanning confocal microscope.Results Bone marrow stroma cells had good affinity on air/jet electrospinning silk fibroin nanofiber scaffolds.The cells adhered on the surface of nanofiber firmly within 4 h,and there were some cells spreading on the scaffolds.The ratio between spreaded cells and adhered cells was 75.9%,while the ratio was only 49.63% in control group. Conclusion Air/jet electrospinning silk fibroin nanofiber scaffolds have good biocompatibility,and may be a good candidate for the biomedical applications in the bone tissue engineering.