| Ibotenate脑内注射诱导脑白质损伤的新生小鼠模型研究 |
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| 引用本文： | 王爱萍,贺湘英,莫亚雄,梁琨.Ibotenate脑内注射诱导脑白质损伤的新生小鼠模型研究[J].昆明医学院学报,2010,31(1):15-18. |
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| 作者姓名： | 王爱萍 贺湘英 莫亚雄 梁琨 |
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| 作者单位： | 昆明医学院第一附属医院,云南,昆明,650032 |
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| 基金项目： | 云南省自然科学基金资助项目(2004C0019R) |
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| 摘 要： | 目的通过脑内注射神经兴奋毒素鹅膏蕈氨酸（ibotenateacid，IA）建立新生小鼠脑白质损伤模型，为新生儿兴奋毒性脑白质损伤神经保护的研究提供新的实验方法．方法出生后5d的ICR种小鼠60只。随机分为IA组和生理盐水（normalsodium，NS）组．IA组脑内注射神经兴奋毒素IA后随机分为IA1（24h）和IA2（120h）组，Ns组脑内注射等量生理盐水后随机分为Ns1（24h）组和NS2（120h）组．分别于脑内注射后24h和120h断头处死小鼠，观察脑组织病理学，计算各组脑组织的损伤直径．结果IA组小鼠脑组织病理组织切片显示明显的脑白质区囊性病变．IA2组脑损伤直径为（516．7±123.3）μm，IA2组脑损伤直径为（1400±153．2）μm；NS1组脑损伤直径为（250．7±119．5）μm，NS：组脑损伤直径为（382±118．8）μm．IA组脑损伤直径明显大于Ns组（P〈0．01）．结论脑内注射神经兴奋毒素鹅膏蕈氨酸能够造成小鼠明显的脑室周围白质区囊性病变，复制新生小鼠兴奋毒性脑损伤模型
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| 关 键 词： | Ibotenate 脑白质损伤 新生小鼠 |
Intracerebral Injection of Ibotenate Induces the Model of White Matter Damage in Neonatal Mice |
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| WANG Ai-ping,HE Xiang-ying,MO Ya-xiong,LIANG Kun.Intracerebral Injection of Ibotenate Induces the Model of White Matter Damage in Neonatal Mice[J].Journal of Kunming Medical College,2010,31(1):15-18. |
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| Authors: | WANG Ai-ping HE Xiang-ying MO Ya-xiong LIANG Kun |
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| Institution: | Dept.of Pediatrics/a>;The 1st Affiliated Hospital of Kunming Medical University/a>;Kunming Yunnan 650032/a>;China |
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| Abstract: | Objectives To provide a model of the white matter damage in neonatal mice brain through in-tracerebral injection of ibotenate and provide a newexperimental method for neonatal excitotoxic white matter dam-age protection.Methods Sixty 5-day-old ICR neonatal mice were randomly divided into two groups:ibotenate acid(IA)control(n=30)and normal sodium(NS)control(n=30).After intracerebral injection of ibote-nate in group IA,the pups were divided in two sub-groups at random,group IA1 and group IA2.Similarly,after ... |
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| Keywords: | Ibotenate Excitotoxic damage Neonatal mice |
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