细菌质粒提取方法的研究

为建立一种革兰氏阴性及阳性菌均能分析的质粒提取方法，以Biswajit saha的方法为基础，对比酸酚法、热变性法、碱变性法、酶法等方法，研制符合国内一般实验室的方法，结果：按Biswajit saha法Ecol-iv517的8条带在本实验室条件下不能安全检出，但自研方法重复12次均能检出，且与标准蛋白Market的分子量相符合，另取临床分离菌株，金黄色葡萄球菌、大肠埃希氏菌、铜绿假单胞菌各30株，均检出，最大质粒分子量检出186.3kb，最小分子量检出1.1kb，3次重复检测质粒普均相同，结果表明：改良质粒质量提取方法适用于革兰氏阴性及阳性菌大小分子量的质粒提取，便于在一般实验室推广应用。

The Study on Improving Method of Extracting Bacteria Plasmid

Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity． Result The eight strip s of Ecliv 5l7 can not be found completely in original method, but can be found in our one．We found the largest plasmid molecular weight was l86.3?kb, and t he minimum was l.l?kb from each 30 strains STA, EC, PA． Plasmid profile is alike through repeating ex p eriment three times． Conclusion:The method can be widely used in extracting bac teria plasmid and fit gram-negative and gram-positive bacteria whenever molecular weight ma x or min． These conditions can be obtained in general laboratory．