去整合素防治兔眼后发性白内障的实验研究

目的 探讨去整合素DTY29对体外人晶状体上皮细胞及体内兔眼品状体上皮细胞增殖抑制的有效性及其安全性.方法 （1）体外：将36孔板分为a对照组、b、c、d实验组,每组9孔,培养人品状体上皮细胞,细胞划痕后各组依次加入培养液、0.08μmol/L、0.4 μmol/L和2μmol/L浓度DTY29,于0h、6h、12h、24 h倒置显微镜拍照并测量划痕宽度; （2）体内：雄性新西兰大白兔36只,随机分为对照组A组,实验组：B、C、D组,每组9只兔.术前均测角膜内皮细胞计数.右眼均行透明晶状体皮质超声乳化吸除术,术毕A组前房注入林格氏液0.2 mL,B、C、D组分别注入10 μmol/L、20 μmol/L和40 μmol/L DTY29溶液0.2 mL,术后观察角膜、前房、瞳孔、囊膜浑浊及眼底情况.结果 （1）体外细胞划痕实验：6h、12h、24 h划痕宽度比较,a组较b、c、d组均明显缩小（P＜0.05）; （2）角膜内皮细胞丢失A、B、C组间无统计学意义（P＞0.05）,D组与A、B、C组相比,显著减少（P＜0.05）; （3）A、B组术后1～3 m均有后发性白内障发生,但发生率无统计学意义（P＞0.05）,C、D组未发生后发性白内障; （4）各组均未发生视网膜脱离、脉络膜脱离等严重并发症.结论 DTY29体外或体内均能抑制晶状体上皮细胞增生,其抑制作用与药物浓度呈正相关,在活体内,高浓度DTY29对角膜内皮细胞具有破坏作用,暂未发现其对眼内其他组织造成明显影响,证明低浓度下用药的安全性;最适宜的用药浓度可能在10 μmol/L-20 μmol/L之间,其眼内用药安全性和最适宜浓度仍需进一步探索。

Inhibition Effect of Disintegrin on Posterior Capsular Opacification in Rabbits

Objective To investigate the efficacy and safety of disintegrin DTY29 on inhibiting the proliferation of rabbit lens epithelial cells （LECs） in vitro and in vivo. Methods （1） In vitro： 36-well plates were divided into control group （a） and experimental groups （b, c and d） , nine holes each group. The human LECs were cultured and scratched, and then culture media, 0.08 μmol/L, 0.4 μmol/L, and 2 μmol/L DTY29 were added into 4 groups, respectively. Scratch widths were photographed and measured at 0 h, 6 h, 12 h and 24 h. （2） In vivo： 36 male New Zealand white rabbits were randomly divided into control group （A） , experimental groups （B, C and D） , nine rabbits each group. Amounts of corneal endothelial cells were calculated before operative. Phacoemulsification was performed in all right eyes of the rabbits, and then 0.2 mL of Ringer＇s, 10 μ mol/L, 20 μmol/L and 40 μmol/L DTY29 solution was injected into the anterior chamber of groups A, B, C and D, respectively. Cornea, anterior chamber, pupil, posterior capsular opacification （PCO） , and fundus were observed postoperatively. Results At 6 h, 12 h and 24 h of cells scratch test in vitro, the scratch widths of group a reduced significantly （P 〈 0.05） compared with groups b, c and d. There were no significant differences in corneal endothelial cells loss loss among groups A, B and C （P 〉 0.05） , while a significant reduction was found in group D compared with groups A, B and C （P 〈 0.05） . PCO was observed in both groups A and B after 1-3 months postoperatively, but there was no significant difference in incidence （P 〉 0.05） . PCO hadn＇t occurred in groups C and D. Retinal detachment, choroidal detachment and other serious complications were not observed. Oonclusion Disintegrin DTY29 could inhibit lens epithelial cells proliferation in vitro or in vivo, and there was a positive correlation between inhibiting effect and concentration. In vivo, corneal endothelial cells can be destroyed by DTY29 with