伴冠心病的慢性牙周炎患者龈下茵斑中牙周致病菌的检测

目的 观察伴冠心病(coronary heart disease,CHD)的慢性牙周炎患者与慢性牙周炎患者(chronic periodontitis,cP)龈下菌斑中牙周致病菌的分布.方法 收集50例CP患者及50例CP合并CHD患者的龈下菌斑,提取细菌DNA以巢氏聚合酶链反应(nested polymerase chain reaction,Nested PCR)法检测菌斑中牙龈卟啉单胞菌(porphyromonas gingivalis,pg)、中间普氏菌(prevotella intermedia,Pi)、具核梭杆菌(fusobacterium nucleatum,Fn)、伴放线放线杆菌(actinobacillus actinomycetemcomitans,Aa)、福赛斯坦纳菌(tannerella forsythensis,Tf)、齿垢密螺旋体(treponema denficola,Td),并比较6种微生物在CP及CP合并CHD患者物龈下菌斑中的检出率.结果 CP组细菌检出率为:Pg 98%,Pi 92%,Fn 88%,Aa6%,Tf92%和Td98%;CP合并CHD组细菌检测出率为:Pg 100%,Pi 94%,Fn 80%,Aa 14%,Tf96%和Td98%,2组间6种牙周可疑致病菌检出率差别无统计学意义(P>0.05).结论 CP组及cP合并CHD组的龈下菌斑中6种牙周可疑致病菌的分布无明显差别.

Detection of Periodontopathogens in Subgingival Plaque of Patients with Coronary Heart Disease and Periodontitis

Objective To investigate the prevalence and distribution of periodontopathogens in the subgingival plaque of patients with coronary heart disease(CHD)and periodontitis.Methods 50 subgingival plaque samples of patients with CP and 50 subgingival plaque samples of patients with CP and CHD were collected and their bacteria DNA was extracted.Six periodontal pathogens including Porphyromonas gingivalis(Pg),Prevotella intermedia(Pi),Fusobacterium nucleatum(Fn),Actinobacillus actinomycetemcomitans(Aa),Tannerella forsythensis(Tf)and Treponema denticola(Td) were detected by the Nested polymerase chain reaction(Nested PCR).Results In the CP group,the rates of periodontal bacteria were Pg 98%,Pi 92%,Fn 88%,Aa 6%,Tf 92% and Td 98%;In the CP combined with CHD group,the rates of periodontal bacteria were Pg 100%,Pi 94%,Fn 80%,Aa 14%,Tf 96% and Td 98%.Conclusion The distribution of putative periodontopathogens Pg,Pi,Fn,Aa,Tf and Td is similar in the CP group and CP combined with CHD group.