| MTT法测定低分子姬松茸多糖体外抗肿瘤活性 |
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| 引用本文： | 张春,赵学梅,周丽,牛英才,刘吉成.MTT法测定低分子姬松茸多糖体外抗肿瘤活性[J].齐齐哈尔医学院学报,2009,30(1):1-2. |
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| 作者姓名： | 张春 赵学梅 周丽 牛英才 刘吉成 |
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| 作者单位： | 1. 齐齐哈尔医学院医药科学研究所,161042;黑龙江中医药大学药学院
2. 齐齐哈尔医学院医药科学研究所,161042 |
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| 基金项目： | 国家自然科学基金，黑龙江省自然科学基金 |
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| 摘 要： | 目的用体外培养法探讨低分子姬松茸多糖（a low molecular weight polysaceharide isolated from Agaricus blazei，LMPAB）对人白血病细胞k562生长的抑制作用。方法5、10和20μg·ml^-1浓度的LMPAB处理体外培养的k5624 8h后，采用MTT法检测LMPAB对k562生长的影响。结果LMPAB在体外具有明显的细胞毒性，可抑制k562增殖，且呈剂量依赖性。结论LMPAB在体外可有效抑制人白血病细胞k562的生长。
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| 关 键 词： | 抗肿瘤 姬松茸 多糖 MTT法 |
Detecting the anticancer effects of LMPAB by MTT technique in vitro |
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| ZHANG Chun,et al..Detecting the anticancer effects of LMPAB by MTT technique in vitro[J].Journal of Qiqihar Medical College,2009,30(1):1-2. |
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| Authors: | ZHANG Chun |
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| Institution: | Institute of medicine;Qiqihaer Medical University;Qiqihar 161042 china |
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| Abstract: | Objective To observe the anticancer effects of LMPAB （a low molecular weight poly-saecharide isolated from Agaricus blazei Murill） on growth inhibition in K562 cells. Methods K562 Cell proliferation was analyzed by MTT after treated with LMAPAB （5,10 and 20μg ml^-1） for 48 h. Results LMPAB produced cytotoxie effect onthe cell in vitro and could obviously inhibit proliferation of K562 with dose-dependent relation in vitro. Conclusions LMAPAB can inhibit the proliferation of K562 cell significantly. |
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| Keywords: | Agaricus Blazei Murrill Polysaccharide Antineoplastic MTT assay |
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