| 增加CIK细胞耐药性及杀瘤活性机制的研究 |
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| 引用本文： | 李岩,李岩,李淑艳,王淑英,吴琦,张春晶,高涵,冯丽,富东娜,郭红艳,齐晓丹.增加CIK细胞耐药性及杀瘤活性机制的研究[J].齐齐哈尔医学院学报,2010,31(2):169-170. |
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| 作者姓名： | 李岩 李岩 李淑艳 王淑英 吴琦 张春晶 高涵 冯丽 富东娜 郭红艳 齐晓丹 |
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| 作者单位： | 齐齐哈尔医学院生物化学教研室,161006 |
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| 基金项目： | 黑龙江省教育厅科研基金项目 |
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| 摘 要： | 目的 常规方法培养CIK细胞,将多药耐药基因(mdr1)转入细胞内,观察转染前后其对卡铂的耐药性及杀瘤活性机制进行研究.方法 将mdr1的重组质粒转染到对数生长期的CIK细胞,通过RT-PCR鉴定耐药基因表达;Western blot检测mdr1编码的P-gp蛋白的表达;MTT法检测转染前后CIK细胞对卡铂敏感性的变化.结果 在转染mdr1基因后的CIK细胞中,mdr1 mRNA阳性,P-gp的表达较转染前CIK细胞及转染空质粒的CIK细胞明显增高,差异有统计学意义(P<0.05),转染mdr1基因后的CIK细胞对卡铂的耐药性明显提高.结论 mdr1基因转入CIK细胞后,细胞获得了对卡铂的耐药性.
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| 关 键 词： | 细胞因子诱导的杀伤细胞 多药耐药 基因转染 |
Research on increasing multidrug resistance of Cytokine-induced killer (CIK) cells and mechanism of the anti-tumor activity |
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| SHI Yan,et al..Research on increasing multidrug resistance of Cytokine-induced killer (CIK) cells and mechanism of the anti-tumor activity[J].Journal of Qiqihar Medical College,2010,31(2):169-170. |
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| Authors: | SHI Yan |
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| Institution: | SHI Yan,et al.(Department of Biochemistry,Qiqihar Medical University,Qiqihar,Heilongjiang 161006,P.R.China) |
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| Abstract: | Objective To investigate the multidrug resistance to Carboplatin,the Cytokine-inducedkiller(CIK) cells transfected with the multidrug resistance(mdrl) c DNA. Methods CIK cells were induced by culturing PBMC with regular method.CIK cells were transfected with human mdrl cDNA recombinant plasmid,RT-PCR was used to detect mdrl mRNA in transfected CIK cells.P-glycoprotein(P-gp) expression of CIK cells was assayed by western blotting.Multidrug resistance to Carboplatin were performed by MTT method.Results Mdrl m... |
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| Keywords: | Cytokine induced killer(CIK) Multidrug resistance Gene transfection |
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