芹菜素对大鼠缺血再灌注肝脏的保护作用

目的观察芹菜素对大鼠缺血再灌注肝脏损伤的防护作用。方法雄性SD大鼠,随机分为假手术组、缺血再灌注组(I/R组)和I/R加芹菜素处理组。肝脏缺血再灌注后,分别观察血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)及乳酸脱氢酶(LDH)含量的变化,同时分析肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性及肝组织病理学的改变。结果肝脏缺血再灌注后,与假手术组比较,血清中AST、ALT、LDH及肝组织中MDA含量均显著增加,而SOD活性显著的降低(P<0.01),同时肝脏门静脉周围瘀血明显,可见不同程度的肝细胞肿胀、脂肪空泡变;经芹菜素处理后,与缺血再灌注组相比较,血清中AST、ALT、LDH及肝组织中MDA均降低,而SOD活性增高(P<0.05),同时肝脏的瘀血明显较缺血再灌注组轻,微血管未见明显损伤,窦间隙稍宽,细胞变性坏死不明显。结论芹菜素对大鼠肝脏缺血再灌注损伤有一定的保护作用。

The Protective Effect of Apigenin on Hepatic Ischemia Reperfusion Injury in Rats

Objective To investigate the protective effect and its role of apigenin on hepatic ischemia reperfusion injury in rats.Methods Male Sprague-Dawley rats were randomly divided into three groups: pseudo-operation normal control group, hepatic ischemia reperfusion injury model group, apigenin group.After hepatic ischemia and reperfusion,to analysis the level of ALT,AST and LDH in the serum and observe the change of MDA and SOD activity of the hepatic tissue.The injury circumstance of the liver tissue in each group was compared by HE staining.Results After hepatic ischemia reperfusion,the MDA level and the content of ALT,AST and LDH increased,and the SOD activity decreased obviously comparing with the normal group (P<0.01); Comparing to the hepatic ischemia reperfusion injury model group,the MDA level and the content of ALT,AST and LDH decreased,and the SOD activity increased (P<0.05).The change could be also observed by the optical microscope which liver cell injury of the apigenin groups was evidently slight than that of the hepatic ischemia reperfusion injury model group.Conclusions The apigenin have a prominent protective effect on hepatic ischemia reperfusion injury in rats.