脱氢乙酸小鼠骨髓细胞微核试验

目的：采用小鼠骨髓细胞微核试验，对脱氢乙酸进行体内致突变安全性评价。方法按照1/10 LD50、1/5 LD50和1/2 LD50设置三个剂量组，另设置阴性对照组和环磷酰胺（ CP）阳性对照组（100 mg·kg-1）。每组10只小鼠，雌雄各半，灌胃给药，两次攻毒时间间隔24 h。在第二次灌胃后6 h，处死小鼠，制片，观察并计数嗜多染红细胞的微核率，并与阳性对照组和隐形对照组进行比较分析。结果1/5 LD50、1/10 LD50剂量时，与正常对照组无显著差异；1/2 LD50剂量组与正常对照组有显著差异（ P﹤0.05），但无剂量-效应关系。结论脱氢乙酸小鼠骨髓细胞微核试验结果为阴性。

Micronucleus test of dehydroacetic acid in mice

Objective To investigate mutational function of dehydroacetic acid（ DHA）. Methods The experimental animals were randomly divided into five groups such as negative control group,1/10 LD50 ,1/5 LD50 ,1/2 LD50 and positive control group,respectively. Ten animals were in each group,half male and female. The mice received DHA through gavage by 30 h method（24 h interval between 2 times）. Sliders were made,polychromatic erythrocyte（PCE）was observed and rates of their micromucleus were counted an compared with positive and negative groups. Results Beside of 1/5 LD50 ,1/10 LD50 DHA groups,the 1/2 LD50 group was obviously different from normal mice（P﹤0. 05）,but there were not dose-effect relationship within three doses of DHA groups. Conclusion The micronucleus test of DHA were observated a nega-tive result.