| 逆行性胰胆管注射法制作重症急性胰腺炎大鼠模型 |
| |
| 引用本文： | 杨波,黄鹤光,陈大良,殷凤峙.逆行性胰胆管注射法制作重症急性胰腺炎大鼠模型[J].福建医科大学学报,2002,36(1):71-72,I003. |
| |
| 作者姓名： | 杨波 黄鹤光 陈大良 殷凤峙 |
| |
| 作者单位： | 福建医科大学,附属协和医院普外科,福州,350001 |
| |
| 基金项目： | 福建省教育厅科研基金资助项目 ( JA9810 6 ) |
| |
| 摘 要： | 目的 建立一种重复性好，标准化程度高，病理变化典型，适合评价药物疗效的重症急性胰腺炎（SAP）动物模型。方法 28只大鼠逆行胰胆管注射牛磺胆酸钠，观察死亡率，血淀粉酶，血Ca^2 和胰腺组织病理结构变化。结果 与假手术组相比，SAP组血淀粉酶升高（P＜0．01），血Ca^2 降低（P＜0．05），部分大鼠死亡（63．1％），病理检查胰腺组织明显坏死。结论 采用逆行胰胆管注射法建立SAP大鼠模型具有重要性好，标准化程度高，病理变化典型等优点，适合重症急性胰腺炎发病机制和药物疗效的研究。
|
| 关 键 词： | 动物疾病模型 胰腺炎 急性病 大鼠 |
| 文章编号： | 1000-2235(2002)01-0071-02 |
Severe Acute Pancreatitis Rat Model Induced by Retrograde Cholangiopancreatic Duct Infusion of Bile Salt |
| |
| YANG Bo,HUANG He guang,CHEN Da liang,et al.Severe Acute Pancreatitis Rat Model Induced by Retrograde Cholangiopancreatic Duct Infusion of Bile Salt[J].Journal of Fujian Medical University,2002,36(1):71-72,I003. |
| |
| Authors: | YANG Bo HUANG He guang CHEN Da liang |
| |
| Abstract: | Objective To establish an experimental model of severe acute pancreatitis induced by retrograde cholangiopancreatic duct infusion of bile salt. Methods Severe acute pancreatitis(SAP) was induced by retrograde cholangiopancreatic infusion of sodium taurocholate in 8 rats, with a sham operation group(n=8) used as control. The concentration of serum calcium, the activity of serum amylase were measured. The histological appearances of the pancreas were also observed. Results Compared with the sham operation group, the activity of serum amylase and the mortality were increased. Serum calcium was decreased in SAP group. Pancreatic ledema, haemorrhage, acinar cell necrosis and extensive fat necrosis were found in the pancreas of SAP group. Conclusion The experimental model of severe acute pancreatitis induced by retrograde cholangiopancreatic duct infusion of bile salt can be a useful tool to investigate the pathogenesis and treatment of severe acute pancreatitis. |
| |
| Keywords: | disease model animal pancreatitis acute disease rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
