热休克蛋白70在瑞芬太尼后处理减轻大鼠肝脏缺血再灌注损伤中的作用

目的 探讨热休克蛋白70（HSP70）在瑞芬太尼后处理减轻大鼠肝脏缺血再灌注损伤（HIRI）中的作用。 方法 将24只SD大鼠随机分为4组（n＝6）：假手术组（S组）、HIRI组（I/R组）、瑞芬太尼组（R组）和槲皮素组（Q组）。制备大鼠HIRI模型。R组再灌注开始10 min内给予瑞芬太尼4 g/kg；Q组在给予瑞芬太尼前先给予槲皮素7 mg/kg。再灌注结束检测血清丙氨酸氨基转移酶（ALT）和天门氨酸氨基转移酶（AST）活性以及肝组织超氧化物歧化酶（SOD）活性、丙二醛（MDA）和HSP70含量。 结果 与S组比较，其余3组血清ALT、AST活性和肝组织MDA、HSP70含量均升高，而肝组织SOD活性I/R组和Q组降低，R组升高（P＜0.05）。与I/R组比较，R组血清ALT、AST活性和MDA含量降低，而肝组织SOD活性和HSP70含量升高（P＜0.05）。 结论 瑞芬太尼后处理可减轻大鼠HIRI的急性肝损伤，抑制肝脏脂质过氧化反应，其作用机制与增强肝组织HSP70表达有关。

Role of heat shock protein 70 in remifentanil post-treatment-induced reduction of hepatic ischemia-reperfusion injury in rats

Objective To explore the effect of heat shock protein 70(HSP70) on remifentanil-post-treatment-induced hepatic ischemia-reperfusion injury (HIRI) in rats. Methods Twenty-four Sprague-Dawley rats were randomly divided into four groups( n = 6 ): sham-operation group (group S), HIRI group ( group I/R) , remifentanil group(group R) and quercetin group (group Q).Rat HIRI model was prepared. In Group R, remifentanil 4 ug·kg-1 was given at the beginning of reperfusion within 10 minutes. In group Q, quercetin 7 mg·kg-1 was given before remifentanil treatment. The activities of serum alanine transaminase(ALT) and aspartate transaminase(AST) were measured after reperfusion, and the activity of superoxide dismutase(SOD) , the content of malondialdehyde (MDA ) and HSP70 in liver tissue were measured. Results Compared with group S,?serum ALT and AST activity , liver MDA and HSP70 content were increased in the other three groups, while SOD activity in group I/R and group Q were decreased and that in group R was increased ( P<0.05) .Compared with group I/R, serum ALT,AST activity and MDA content in group R were decreased, while liver SOD activity and HSP70 content were increased( P<0.05). Conclusion Remifentanil post-treatment can attenuate the acute liver injury induced by HIRI and inhibit the lipid peroxidation response, which is related to increasing the expression of HSP70 in liver.