孕酮对大鼠颌下腺表皮生长因子及其受体表达的影响

目的 探讨孕酮对大鼠颌下腺表皮生长因子（ＥＧＦ）及其受体（ＥＧＦＲ）表达的影响。方法 用免疫组化方法观察注射１，３，５和７天孕酮后，成年雌性ＳＤ大鼠颌下腺ＥＧＦ、ＥＧＦＲ表达及颗粒曲管（ＧＣＴ）形态的改变。结果 与对照组比较，注射孕酮１天组，ＥＧＦ的阳性表达最强，３天组较强，５天组减弱，７天组复又增强；ＥＧＦＲ的表达１，３天组减弱，５天组增强，７天组复又减弱，与ＥＧＦ的表达基本相反。ＧＣＴ最宽径及

Influence of Progesterone on Epidermal Growth Factor and its Receptor Expression in Submandibular Gland of Rats

Objective\ To investigate the effect of progesterone on epidermal growth factor(EGF) and EGF receptor(EGFR) expression in submandibular gland of rats.\ Methods\ With immunohistochemical SP method,EGF and EGFR were investigated and the morphological parameters of granular convoluted tubule(GCT) were measured respectively in submandibular gland(SMG) of adult female SD rat injected by progesterone for 1, 3, 5 and 7 days(d).\ Results\ Compared with the untreated group,the expression of EGF,after the injection of progesterone,increased sharply and obviously in group 1 d and group 3 d,decreased in group 5 d ,and then increased again in group 7 d.\ But the expression of EGFR after the injection,decreased in group 1 d and in group 3 d ,increased in group 5 d and then decreased again in group 7 d.\ So it was contrary to that of EGF.\ The change of the diameter of GCT and the height of its cells was almost the same as that of EGFR or in a delayed fashion.\ Conclusion\ That progesterone would enhance the GCT cells in SMG of rats to synthesize EGF.\ Down regulation lies between EGF and EGFR,the change of the GCT is related to the amount of EGFR.