人外周血树突细胞的分离与提纯

目的 从人外周血中分离、培养及鉴定树突细胞（ＤＣ）。方法 从正常人外周血分离获得单个核细胞，培养２小时后，取会细胞，在无血清培养基中加入不同的细胞在子，于２的第１，６，８天对形态，表型和功能分擀行测定，并在光镜、电镜下观察ＤＣ的纯度与得率。结果 体外培养６～８天可获得高纯度大量的ＤＣ，较高表达ＨＬＡ ＤＲ、ＣＤ４０、ＣＤ８３和ＣＤ８６，能强烈激发同种慢体Ｔ淋巴细胞增殖。结论 上述方法可以从人外周血

Isolation and Purification of Dendritic Cells from Human Peripheral Blood

Objective\ Proliferation and isolation of dendritic cells from human peripheral blood.\ Methods\ Monocytes isolated from human peripheral blood, culture for 2 hours.\ The adherent cells were cultured with different cytokines respectively in serum\|free culture.\ The cells were examined by phenotype/FACS for 1,6 and 8 days respectively,and the cell growth was inspected under a light microscope and electronmicroscope at regular interval and determined function assay.\ Results\ During 6 days to 8 days, a large number of DC with high purity were generated.\ DC expressed HLA\|DR,CD\-\{40\},CD\-\{86\} and CD\-\{83\} costimulating moleculars highly on cell surface and could stimulate proliferation of allogeneic T lymphocytes.\ Conclusion\ In serum\|free culture,a numbers of dendritic cells was obtained from the human peripheral blood.