茶多酚对心脏骤停大鼠复苏后海马神经元损伤及凋亡的影响

目的:观察茶多酚对心脏骤停大鼠复苏后海马神经元损伤及凋亡的影响。方法:选择SD大鼠18只,随机分为3组:假手术组(n=6)、盐水组(n=6)、茶多酚组(n=6)。盐水组及茶多酚组大鼠麻醉、插管后经食道起搏诱导心脏骤停,常规心肺复苏,自主循环恢复即刻分别给予静脉泵入生理盐水2mL/kg、茶多酚10mg/kg,监测大鼠1h后结扎血管放入笼内饲养。假手术组大鼠仅行麻醉、插管,未诱导心脏骤停,监测1h后结扎血管放入笼内饲养。自主循环恢复后48h处死大鼠取出海马组织,行HE染色观察病理形态学改变及TUNEL方法测定神经元凋亡情况。结果:茶多酚组大鼠海马区病理损伤及神经元凋亡程度均低于盐水组,盐水组的凋亡指数为(27.1±11.9)%,茶多酚组的凋亡指数为(4.9±2.2)%,二者比较差异有统计学意义(P<0.01)。结论:茶多酚可减轻复苏后大鼠海马区病理损伤及凋亡程度,对神经元有一定保护作用。

EFFECTS OF TEA POLYPHENOL ON NEURONAL INJURY AND APOPTOSIS IN THE HIPPCAMPAL REGION OF RATS AFTER CARDIOPULMONARY RESUSCITATION

Objective: To observe the influence of tea polyphenol(TP) on neuronal injury and apoptosis in the hippocampal region of rats after cardiopulmonary resuscitation(CPR).Methods: Eighteen healthy Sprague Dawley rats were randomly assigned to sham control,saline and TP groups(n=6;respectively).The rats in saline and TP groups were anesthetized and intubated,and then they were induced to cardiac arrest by trans-esophageal cardiac pacing.They were given with intravenous fluid infusion of TP 10 mg/kg or saline 2 mL/kg as soon as restoration of spontaneous circulation(ROSC).The rats were kept in cages with vascular ligation after 1 h observation.The sham control group was anesthetized and intubated,but cardiac arrest was not induced.They were kept in cages with vascular ligation after 1 h observation.The pathological changes of the rats in the hippocampal region were observed by HE staining,and cell apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP nick end labeling(TUNEL) method at 48 h point after ROSC.Results: The pathological changes and the amount of apoptotic cells in the hippocampal region were higher in saline group than in TP group.The apoptosis index was different between TP and saline groups (4.9±2.2)% vs(27.1±11.9)%,P<0.01).Conclusion: TP administration could decrease the degree of injury and apoptosis in the hippocampal region after CA/CPR,and had protective effects on neurons.