融水苗族STR基因座D7S820、D13S317、D16S539的遗传多态性研究

目的：为了解广西融水苗族人群无关个体的3个短串联重复序列（short tandem repeat,STR）：D7S820、D13S317、D16S539遗传多态性分布情况.方法：用枸橼酸钠抗凝法采集融水苗族208份无亲缘关系的健康个体的血样（男102例,女106例）,酚-氯仿抽提法提取DNA,应用荧光标记复合扩增技术对血样DNA的3个STR基因座进行扩增,用ABI3100型遗传分析仪对扩增产物进行检测.结果：3个STR位点共检测出21种等位基因,62种基因型,频率分布分别在0.004 8～0.448 4和0.004 8～0.201 9之间,其中累积个人识别力0.998 6,累积非父排除率0.956 6,多态信息总量0.972 9;基因型的分布符合Hardy-Weinberg平衡定律（P＞0.05）.结论：苗族群体的3个STR位点具有高度多态性和遗传稳定性,符合孟德尔遗传规律,可以应用于个体识别和亲权鉴定,可作为广西苗族的民族遗传学研究和法医学鉴定的基础数据.

STUDY ON POLYMORPHISMS OF 3 STR LOCI IN MIAO ETHINC GROUP OF GUANGXI RONGSHUI

Objective: To investigate the distribution of three short tandem repeat (STR) loci in Miao ethnic group of Guangxi's Rongshui County. Methods: We collected the Sodium citrated-blood specimens from 208 (Man:102, female: 106) healthy unrelated Miao individuals in Guangxi's Rongshui County, and used the phynol-chloroform method to extracte DNA. By using PCR Amplification Kit and ABI3100 Genetic Analyzer. Result: Totally 21 alleles and 62 genotypes of three STR were observed in 208 healthy unrelated Miao individuals of Guangxi's Rongshui County. The allele frequency and genotype frequency were 0.004 8~0.448 4 and 0.004 8~0.201 9 respectively. Total discrimination power was 0.998 6,cumulative exclusion power 0.956 6,and total polymorphism information 0.972 9; The expected and observed genotype distributions of three loci accord with Hardy-Weinberg equilibrium(P>0.05). Conclusion: the three STR of Miao ethnic group shows high polymorphism and hereditary stability,accord with Mendel's law. The data obtained can be used in population genetics research and forensic application, and individual identification and parental checkup.