BMP-2基因修饰自体BMSCs移植促进兔下颌骨牵张成骨新骨形成的实验研究

目的:检测骨形态发生蛋白-2(BMP-2)基因mRNA及其蛋白的表达,探讨BMP-2基因修饰自体骨髓间充质干细胞(BMSCs)移植对兔下颌骨牵张成骨新骨形成的促进作用。方法:取新西兰白兔36只随机分为3组,每组12只。建立牵张成骨动物模型,在固定期第2天,实验组于牵张间隙注射200μL的BMP-2基因修饰的自体BMSCs液;对照组注射等量自体BMSCs液;空白组注射等量生理盐水。分别于固定2,6周通过逆转录多聚酶链式反应(RT-PCR)、免疫组化等手段检测BMP-2基因mRNA及其蛋白的表达情况。结果:实验组牵张间隙新生骨组织均可见BMP-2基因mRNA和其蛋白强阳性表达。结论:BMP-2基因修饰的自体BMSCs移植能有效促进兔下颌骨牵张成骨新骨形成。

EXPERIMENTAL STUDY ON TRANSPLANTATION OF BMP-2 GENE TRANSFECTED AUTOGENOUS BONE MESENCHYMAL STEM CELLS FOR PROMOTING BONE REGENERATION IN RABBIT MANDIBULAR DISTRACTION OSTEOGENESIS

Objective:To examine the expressions of both bone morphogenetic protein-2(BMP-2) gene mRNA and related proteins and investigate the promotive effect of transplantation of BMP-2 gene transfected autogenous bone mesenchymal stem cells on bone regeneration in rabbit mandibular distraction osteogenesis.Methods: Thirty-six New Zealand’s white rabbits were randomly divided into three groups with twelve in each.All objects were prepared into distraction osteogenesis surgical model on right mandibles.On the 2nd day of consolidation,experimental,control,and blank groups were injected with the same amount of 200 μL of the solution with BMP-2 gene transfected autogenous bone mesenchymal stem cells,the solution with autogenous bone mesenchymal stem cells,and physiological saline at distraction gap,respectively.The expressions of BMP-2 mRNA and related proteins were examined by RT-PCR and immunohistochemistry at the ends of the 2nd and 6th week consolidations,respectively.Results: Strongly positive expression of both BMP-2 gene mRNA and related proteins were confirmed on regenerated bone in distraction gap.Conclusion: The transplantation of BMP-2 gene transfected autogenous bone mesenchymal stem cells could effectively promote bone regeneration in rabbit mandibular distraction osteogenesis.