| 树Qu对丙型肝炎病毒的易感性研究 |
| |
| 引用本文： | 谢志春,高伟志.树Qu对丙型肝炎病毒的易感性研究[J].广西医科大学学报,2000,17(3):347-350. |
| |
| 作者姓名： | 谢志春 高伟志 |
| |
| 作者单位： | 广西医科大学公共卫生学院,南宁 |
| |
| 基金项目： | 国家自然科学基金，39270604， |
| |
| 摘 要： | 目的：探讨树Ｑｕ对丙型肝炎病毒（ＨＣＶ）的易感性。方法：对来自云南的树Ｑｕ接种含ＨＣＶ的病人血清，然后用ＰＣＲ方法检测树Ｑｕ血清的ＨＶＣ ＲＮＡ，用反向被动血凝法测定抗－ＨＣＶ抗体，用原位ＰＣＲ方法检测树Ｑｕ肝细胞内的ＨＶＣ ＲＮＡ。结果：３４．８％的树Ｑｕ（８／２３）在接种ＨＣＶ后，血清中检出ＨＣＶ ＲＮＡ，对４例抗ＨＣＶ阳性和谷丙转氨酶升高的树Ｑｕ的肝组织进行原位ＰＣＲ检测，结果在１例肝细胞中
|
| 关 键 词： | 树Qu 丙型肝炎病毒 原位PCR 丙型肝炎 易感性 |
| 修稿时间： | 2000年1月7日 |
INVESTIGATION OF SUSCEPTIBILITY OF TREE SHREWS TO HEPATITIS C VIRUS |
| |
| Xie Zhichung,Gao Weizhi,Su Jiehan,et al.INVESTIGATION OF SUSCEPTIBILITY OF TREE SHREWS TO HEPATITIS C VIRUS[J].Journal of Guangxi Medical University,2000,17(3):347-350. |
| |
| Authors: | Xie Zhichung Gao Weizhi Su Jiehan |
| |
| Abstract: | Objective:To study the susceptibility of tree shrews to hepatitis c virus (HCV)Methods:The blood of tree shrews obtained from Yunan China and inoculated with HCV was examined for the presence of HCV RNA by PCR and antiHCV antibody by passive hemagglutination assayIn addition,liver tissue obtained from serum HCV RNA positive tree sherw was analyzed by in situ PCRResults:348% of tree shrews(8/23) developed viremia and 304% (7/23) presented antiHCV antibody after inoculationHCV RNA was also found in the hepatocytes of a tree shrew whose serum was positive for HCV RNAHowever,neither HCV RNA nor antiHCV was found in control tree shrewsAlanine aminotransferase (ALT) was elevated in some infected tree shrews and one control animalConclusion:Tree shrew is susceptible to HCV |
| |
| Keywords: | tree shrew hepatitis c virus PCR in situ PCR |
| 本文献已被 CNKI 维普 等数据库收录！ |
