胶原支架材料复合膜的制备及其组织培养性能观察

目的 制备纯胶原膜、胶原／HA膜、胶原／甲壳糖膜和胶原／透明质酸／甲壳糖膜(简称胶原复合膜)，并研究其组织培养性能。方法 分别测定其体外降解时间和细胞增殖数。结果 (1)降解时间为：纯胶原膜1380min、胶原／HA膜1376min、胶原复合膜1560min。(2)在胶原／HA膜、胶原复合膜上，增殖细胞的数目一直是最大的。结论 (1)加入甲壳糖有利于降低胶原膜体外降解速度，而透明质酸没有。(2)加入透明质酸有利于细胞在胶原膜上的吸附、生长、增殖，而甲壳糖没有。

Preparation of a composite membrane with collagen scaffold material for tissue culture]

OBJECTIVE: To prepare a composite membrane with collagen scaffold material for tissue culture. METHODS: Pure collagen membrane, collagen/hyaluronic acid (HA) membrane, collagen/chitosan membrane and collagen/chitosan/ HA membrane (abbreviated as composite collagen membrane) were prepared respectively, and their respective biodegradation time and the number of the cells proliferated on them were examined in vitro. RESULT: The biodegradation time of pure collagen, collagen/HA and composite collagen membranes in vitro was 1 380, 1 376 and 1 560 min respectively, and the number of cells proliferated on the collagen/HA membrane and the composite collagen membrane were greater than that on the pure collagen membrane. CONCLUSIONS: Addition of chitosan can decrease the biodegradation of the collagen membrane in vitro, while HA can not; HA Can contribute to cell adhesion and proliferation on the collagen membranes, but chitosan does not.