| 经癌胚抗原重组痘苗病毒转染的树突状细胞体外诱导特异性T细胞免疫 |
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| 引用本文： | 吴军,杨太成,等.经癌胚抗原重组痘苗病毒转染的树突状细胞体外诱导特异性T细胞免疫[J].第一军医大学学报,2002,22(3):256-258. |
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| 作者姓名： | 吴军 杨太成 |
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| 作者单位： | [1]广州军区广州总医院放射肿瘤科 [2]广州军区广州总医院肿瘤分子生物学研究所 |
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| 摘 要： | 目的 研究人癌抗原重组痘苗病毒（rV－CEA）转染上周血树突状细胞（DC）后能否在体外诱导CEA特异性细胞性T淋巴细胞免疫。方法 将rV－CEA转染外周血单核细胞来源的DC后用于激发同源的T细胞，检测其对T细胞的增殖作用以及对CEA分泌性肿瘤细胞的杀伤活性，并与未经rV－CES转染的DC激发的T细胞进行比较。结果 经rV－CEA转染的DC激活的T细胞对CEA分泌性肿瘤细胞具有特异性杀伤作用。结论 rV－CEA转染的DC可以诱导CEA特异性T细胞活性。
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| 关 键 词： | 树突状细胞 癌胚抗原 痘苗病毒 T淋巴细胞 细胞增值 基因转染 rV－CEA |
Dendritic cells transfected with carcinoembryonic antigen-vaccinia recombinant virus induces CEA-specific immunity mediated by cytotoxic T lymphocytes in vitro.] |
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| Jun Wu,Xiao-Huai Wang,Tai-Cheng Yang,Jiang Xian,Wen-Ling Zheng.Dendritic cells transfected with carcinoembryonic antigen-vaccinia recombinant virus induces CEA-specific immunity mediated by cytotoxic T lymphocytes in vitro.][J].Journal of First Military Medical University,2002,22(3):256-258. |
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| Authors: | Jun Wu Xiao-Huai Wang Tai-Cheng Yang Jiang Xian Wen-Ling Zheng |
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| Institution: | Department of Oncology, General Hospital of Guangzhou Command, Guangzhou 510010, China. |
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| Abstract: | OBJECTIVE: To observe whether dendritic cells (DCs) transfected with carcinoembryonic antigen (CEA)-vaccinia recombinant virus (rV-CEA) induces cytotoxic T lymphocyte-mediated CEA-specific immunity in vitro. METHODS: DCs derived from peripheral blood monocytes were transfected with rV-CEA and then cocultured with autologous T cells. The proliferation of the induced T cells and their cytotoxic activity against CEA-secreting tumor cells were assessed and compared with those of T cells induced by DCs without rV-CEA transfection. RESULTS: T cells induced by DCs transfected with rV-CEA showed specific cytotoxicity against CEA-secreting tumor cells. CONCLUSION: DCs transfected with rV-CEA can elicit activation of CEA-specific T cells. |
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