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动态张应变下大鼠肌卫星细胞三维培养的形态学观察
引用本文:熊绍虎,李宁,赵金梅,廖华,秦廷武,钟世镇.动态张应变下大鼠肌卫星细胞三维培养的形态学观察[J].第一军医大学学报,2003,23(2):141-143.
作者姓名:熊绍虎  李宁  赵金梅  廖华  秦廷武  钟世镇
作者单位:[1]蚌埠医学院附属医院手术室,安徽蚌埠233004 [2]第一军医大学广东省组织构建和检测重点实验室,广东广州510515 [3]四川大学华西医院骨科,四川成都610041
摘    要:目的 探讨三维培养条件下动态张应变对大鼠骨骼肌卫星细胞形态学的影响。方法 将牵张应变(10%应变、频率0.2Hz,每小时加载10min,共作用48h)作用于大鼠骨骼肌卫星细胞—支架材料复合物。结果 在动态张应变作用下,骨骼肌卫星细胞沿应力方向延伸,并见到肌小管形成;而未施加应力的骨骼肌卫星细胞在各个方向上都有突起,但未见到肌小管形成。结论 动态张应变有利于肌纤维理想极性的形成,并有助于组织工程化骨骼肌的构建。

关 键 词:动态张应变  骨骼肌  卫星细胞  形态学  三维培养  整形修复外科

Morphological study on three-dimensional culture of rat muscle satellite cells under cyclic mechanical stretch]
Shao-hu Xiong,Ning Li,Jin-mei Zhao,Lei Yu,Hua Liao,Ting-wu Qin,Shi-zhen Zhong.Morphological study on three-dimensional culture of rat muscle satellite cells under cyclic mechanical stretch][J].Journal of First Military Medical University,2003,23(2):141-143.
Authors:Shao-hu Xiong  Ning Li  Jin-mei Zhao  Lei Yu  Hua Liao  Ting-wu Qin  Shi-zhen Zhong
Institution:Key Laboratory of Tissue Construction and Detection of Guangdong Province, First Military Medical University, Guangzhou 510515, China. ln-xsh@sohu.com
Abstract:OBJECTIVE: To study the effect of mechanical stretch on the morphologic change of rat muscle satellite cell in the three-dimensional culture. METHODS: Based on the apparatus for three-dimensional cell cultures under a cyclic mechanical strain, a specific stretch pattern (10% elongation, 10 stretches/min for 10 min of each hour 48 h total) was applied to cell-scaffolding composites. RESULTS: Under the stretch pattern, the shape of satellite cells changed to oblate and spread to the direction of the stretch. Furthermore, the myotube was observed. On the contrary, the satellite cells spread to the all direction, and the formation of the myotube was not been found in the control group. CONCLUSION: Cyclic mechanical stretch is helpful for the formation of the ideal directivity and these results are compatible with a significant role for the stretch in tissue-engineered muscle construction.
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