成肌调节因子MyoD与myogenin在肌肉损伤修复过程的动态变化

目的 探讨成肌调节因子MyoD与myogenin在肌肉损伤修复过程中的动态表达。方法 用盐酸布比卡因局部注射制作肌肉损伤模型，在损伤后不同时间点取腓肠肌行冰冻切片，HE染色显示损伤肌肉的病理变化，用SABC法检测MyoD与myogenin的表达。结果 MyoD在肌肉损伤后18h开始表达，48h达高峰；myogenin在肌肉损伤后24h开始表达．72h达高峰。结论 MyoD与myogenin在肌肉损伤后的再生修复过程中起作用，可作为鉴定肌肉前体细胞和反映肌肉再生的指标。

Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury]

OBJECTIVE: To observe the dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during the repair of injured muscle. METHODS: Muscular injury model was established by local injection of bupivacain, and at different time points following the injection, the gastrocnemius muscles were collected for preparation of cryosections. HE staining was performed for examination of the pathological changes in the injured muscles, and the expressions of MyoD and myogenin were detected by SABC. RESULTS: MyoD-positive nuclei began to appear 18 h after muscle injury, reaching the peak till 48 h after the injury. Myogenin-positive nuclei appeared 24 h after muscle injury and peaked at 72 h. CONCLUSIONS: MyoD and myogenin play a role in muscle regeneration after muscle injury, and they may serve as the indexes to identify muscle precursor cells and mark muscle regeneration process.