副溶血弧菌不耐热溶血毒素tlh基因的序列分析

目的:利用PCR技术扩增副溶血弧菌不耐热溶血毒素tlh基因，对tlh基因进行生物信息学分析。方法:利用PCR技术扩增tlh基因，克隆至载体pET32a^ 并测序。将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。结果和结论:测序结果tlh14-90被GenBank收录，登录号为AY289609。tlh14-90全长1257bp，与国际标准株WPI的同源性为99%，含有启动子和密码子，预测其编码一个含418个氨基酸的蛋白质(TLHl14-90)，分子式为C2131H3184N548O649S16，相对分子质量47392．9，理论等电点pI为4．92，丙氨酸、亮氨酸和天冬酰氨的含量分别为11．0％、7．4％和7．2％．含有4个半胱氨酸(Cys)，κ—D法推算的疏水性参数为-34。预测其二级折叠结构为α/□蛋白，三级结构未给出。

Bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene]

OBJECTIVE: To carry out bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene (tlh) obtained by PCR amplification. METHODS: The tlh gene amplified by PCR was cloned into the vector pET32a(+) and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet. RESULTS AND CONCLUSIONS: The sequenced tlh gene (named tlh14-90) was entered into GenBank with the accession number of AY289609. Tlh14-90 has a length of 1 257 bp with both start and stop codons, having 99% homology with the tlh gene of WP1. Tlh14-90 is predicted to encode a protein containing 418 amino acids (named TLH14-90, with the molecular formula of C(2131)H(3184)N(548)O(649)S(16), molecular weight of 47 392.9, and the theoretical PI of 4.92). This protein consists of 4 Cys and the contents of Ala, Leu and Asn are 11.0%, 7.4% and 7.2%, respectively, having a hydrophobic parameter of -34 calculated using kappa-D method. Predicted as a alpha/protein for its secondary structure, TLH14-90 has not been identified for its tertiary structure.