| 线栓法大鼠缺血／再灌注脑损伤模型的改良 |
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| 关 键 词： | 缺血 线栓法 再灌注脑损伤 动物模型 大鼠 |
Surgical procedure improvement to produce rat ischemia-reperfusion injury model with intraluminal suture] |
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| Authors: | Hai-shan Jiang Bing-xun Lu Zhong Ji Li-xin Wang Su-juan Zhang |
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| Institution: | Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. icdi_dr@fimmu.com |
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| Abstract: | OBJECTIVE: To describe a modified surgical approach to produce rat ischemia-reperfusion injury model with intraluminal suture. METHODS: After exposure and ligation of the common carotid artery (CCA), the left external carotid artery and pterygopalatine artery were opened in which a 3-0 nylon suture was introduced intraluminally from the distal end of the ligature of the CCA using a scalp needle. Reperfusion injury was induced by withdrawal of the suture. RESULTS: The length of the suture was about 20.0+/-1.8 mm and the success rate of model establishment was nearly 70%. The rats developed typical symptoms and pathological manifestations after the surgery. CONCLUSION: This modified surgical approach is simple for model establishment and does not require special microsurgical skills to ensure the high success rate. |
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