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CD86在大鼠角膜移植排斥反应中的表达及意义
引用本文:白浪,陆晓和,周瑾,党森涛,张进华.CD86在大鼠角膜移植排斥反应中的表达及意义[J].第一军医大学学报,2004,24(5):533-535.
作者姓名:白浪  陆晓和  周瑾  党森涛  张进华
作者单位:[1]第一军医大学珠江医院眼科.广东广州510282 [2]第一军医大学肿瘤研究所,广东广州510515
摘    要:目的 观察大鼠角膜组织中共刺激分子CD86(B7-2)的原位表达,以了解共刺激分子在大鼠角膜移植排斥反应中的作用和意义。方法 制作大鼠角膜移植模型,观察角膜透明度、新生血管。采用免疫组化法检测CD86在角膜、脾脏组织中的表达。结果 术后角膜植片均出现不同程度的新生血管,角膜水肿、混浊,基质增厚。CD86在正常的角膜组织中无阳性表达;在移植后出现急性排斥反应的角膜上皮层中有大量阳性细胞表达。在脾脏的阳性细胞表达与文献报道的一致。结论 共刺激分子CD86在移植后发生排斥的角膜组织中的阳性表达,可能与免疫排斥反应有关。

关 键 词:CD86  大鼠  角膜移植  排斥反应  表达

Immunohistochemical study of in situ CD86 expression in rejected rat corneal graft after penetrating keratoplasty]
Lang Bai,Xiao-he Lu,Jin Zhou,Sen-tao Dang,Jin-hua Zhang.Immunohistochemical study of in situ CD86 expression in rejected rat corneal graft after penetrating keratoplasty][J].Journal of First Military Medical University,2004,24(5):533-535.
Authors:Lang Bai  Xiao-he Lu  Jin Zhou  Sen-tao Dang  Jin-hua Zhang
Institution:Department of Ophthalmology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Abstract:OBJECTIVE: To investigate the expression of CD86 co-stimulatory molecules in rejected rat corneal graft in situ after penetrating keratoplasty. METHODS: Rat models of orthotopical corneal transplantation were established, and after the occurrence of graft rejection, immunohistochemical staining was performed on the corneal whole-mounts and the spleen tissue of the recipient rats. RESULTS: CD86(+) cells were detected in the epithelium of the corneal graft during allograft rejection but not in normal cornea. CONCLUSION: In situ expression of costimulatory molecules in the corneal allograft may be related to the acute immune rejection after penetrating keratoplasty.
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