抗氧化维生素对大鼠运动能力的影响

目的 探讨抗氧化维生素(维生素E和维生素C)对提高大鼠运动能力的作用。方法 雄性SD大鼠50只，随机分为5组，即安静对照组(C)、力竭对照组(E)、维生素E组(M1)、维生素C组(M2)、维生素E和维生素C联合组(M3)，除C组外均进行4周的反复力竭跑台训练。测定大鼠运动总距离、体质量、股四头肌湿质量、股四头肌线粒体丙二醛(MDA)、游离钙、超氧化物歧化酶(SOD)等指标。结果 Ml组和M3组大鼠运动能力显著强于E组，体质量、总运动距离和单侧股四头肌湿质量显著大于E组，单侧股四头肌湿质量显著大于C组，M3组大鼠体质量显著大于A组，M1组和M3组线粒体游离钙、MDA值显著低于E组，SOD活性显著高于力竭对照组；M2组体质量、运动总距离、单侧股四头肌湿质量与E组无显著差异，但骨骼肌MDA、游离钙、SOD等指标与E组相比有显著差异。结论 单独应用维生素E对反复力竭运动大鼠机体有保护作用，可提高反复力竭运动大鼠的线粒体功能和运动能力，联合应用维生素C可加强这种作用。单独应用维生素C显示了一定的保护反复力竭运动骨骼肌的作用，但不足于提高运动能力。

Effect of antioxidant vitamins on the exercise performance of rats]

OBJECTIVE: To investigate the effect of antioxidant vitamins (vitamin E and vitamin C) on the exercise performance of rats. METHODS: Fifty male SD rats were randomly divided into control group (C), exhausting exercise control group (E), vitamin E group (M1), vitamin C group (M2) and vitamin E plus vitamin C group (M3). The rats in the exercising groups (E, M1, M2, M3) were propelled for repeated exhausting runs on the treadmill for 4 weeks. RESULTS: Exclusive use of oral vitamin E or in combination with vitamin C significantly improved the body mass, total exercise treadmill length and net mass of rat quadriceps femoris after the 4-week exercise. No difference was noted between the rats taking oral vitamin C or E alone. The rats in M1, M2 and M3 groups had lower malondialdehyde (MDA) and free calcium content in the quadriceps femoris than the control rats, and SOD activities in the quadriceps femoris mitochondria of rats in the former 3 groups were significantly higher than those of the control group. CONCLUSIONS: Vitamin E can protect the mitochondria in the skeletal muscles and improve the exercise performance of rats, the effect of which can be enhanced by vitamin C, but vitamin C alone can not sufficiently achieve the effects.