| 睡眠剥夺大鼠脑组织氨基酸类神经递质含量的影响 |
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| 摘 要： |
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| 关 键 词： | 睡眠剥夺 脑化学 氨基酸神经递质类 GABA 谷氨酸 |
Effects of sleep deprivation on gamma-amino-butyric acid and glutamate contents in rat brain. |
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| Authors: | Sheng-Xu Wang Qiu-Shi Li |
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| Institution: | Department of Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | OBJECTIVE: To investigate the effects of sleep deprivation on the contents of gamma-amino-butyric acid (GABA) and glutamate (GLU) in rat brain. METHODS: Thirty rats were assigned into sleep-deprivation (SD, n=10) group (subjected to treatment on small platforms 6.5 cm of diameter), tank control (TC, n=10) group (treated on large platforms 14 cm of diameter) and control group kept in cages (CC, n=10). Rats in SD group were deprived of rapid eye movement sleep for 96 h by "flower pot" technique. High-performance liquid chromatography was used to determine the GABA and GLU contents in the 3 brain regions of the rats: the frontal cortex, hypothalamus and brain stem. RESULTS: The rats in SD group showed an increase in the contents of GABA and lowered ratio of GLU/GABA in all the 3 brain regions (P<0.01) compared with the rats in CC group; GLU contents were also increased in the brain stem and hypothalamus of the rats in SD group (P<0.05). No significant changes were found in TC group. CONCLUSION: Rapid eye movement sleep deprivation induces significant changes in the contents and proportion of amino-acid neurotransmitter in rat brain. |
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