补肾壮骨胶囊对兔膝骨关节炎软骨ADAMTS-4表达的影响

目的:观察补肾壮骨胶囊对兔膝骨性关节炎软骨细胞ADAMTS-4表达的影响。方法:将新西兰白兔40只分为A正常组、B模型组、C治疗组、D对照组,B、C、D 3组按照Hulth造模方法造成兔膝骨性关节炎模型。C组给予补肾壮骨胶囊,D组给予壮骨关节丸。给药8周后测定关节软骨ADAMTS-4阳性细胞数。结果:正常组软骨很少表达ADAMTS-4,模型组中的ADAMTS-4表达显著高于正常组(P<0.01),治疗组和对照组的ADAMTS-4表达显著低于模型组(P<0.01),但高于正常组(P<0.01)。结论:补肾壮骨胶囊抑制KOA软骨中ADAMTS-4的表达。

Effects of Bushenzhuanggu Capsule on Rabbit Knee Osteoarthritis Chondrocytes ADAMTS-4 Expression

Objective:To observe effects of Bushenzhuanggu capsule(BSZGC)on rabbit knee osteoarthritis chondrocytes ADAMTS-4 expression.Methods:In the experiment study,40 New Zealand rabbits were randomly divided into 4 groups:Group A(the normal group),Group B(the model group),Group C(the treated group)and Group D(the control group).KOA models were created in Group B,C and D by Hulth’s operation methods.The rabbits in Group D were treated with Zhuangguguanjie pill(ZGGJP)and BSZGC was used in Group C.8 weeks later,measured ADAMTS-4 positive cells of the rabbits articular cartilage.Results:Normal cartilage rarely expressed ADAMTS-4,the model group of ADAMTS-4 expression was significantly higher than the normal group(P<0.01),treatment group and control group of ADAMTS-4 expression was significantly lower than model group(P<0.01),but higher than the normal group(P<0.01).Conclusion:Bushenzhuanggu capsule inhibit ADAMTS-4 expression of cartilage.