小鼠肝炎病毒温病湿热证模型血清Th1/Th2细胞因子变化研究

目的:以MHV-A59和大肠杆菌作为生物致病因子,建立两种小鼠温病湿热证动物模型,从Th1/Th2探讨温病湿热证的部分致病机制。方法:①温病湿热证的致病机制:动物随机分为3组:正常组、模型组、肠菌组,ELISA法检测小鼠血清IL-4、IFN-γ水平;②造模因素在MHV-A59温病湿热证致病机制形成中的地位:动物随机分为4组:正常组、模型组、湿热组(肥甘饮食+湿热外环境造模)、病毒组(单纯MHV-A59造模),ELISA法检测小鼠血清IL-4、IFN-γ水平;③清热祛湿类方药的对温病湿热证机制的反证:动物随机分为5组:正常组、模型组、A药组(模型组用蒿芩清胆汤治疗)、B药组(模型组用黄连解毒汤治疗)、C药组(模型组用三仁汤治疗),ELISA法检测小鼠血清IL-4、IFN-γ水平。结果:①模型组、肠菌组血清Th1/Th2(IFN-γ/IL-4)比值较正常组明显升高(P<0.05或P<0.01)。②病毒因素主要影响到IFN-γ水平,致使模型组Th1/Th2比值较正常组明显升高(P<0.05)。③与模型组比较,A药组Th1/Th2比值明显趋于平衡(P<0.05)。结论:Th1/Th2失衡是温病湿热证共同的致病机制之一,在MHV-A59温病湿热证形成中,MHV-A59因素主导T细胞免疫紊乱,清热祛湿类方药具有不同程度改善Th1/Th2失衡的作用,反证了温病湿热证的上述致病机制。

Study on Level Changes of Th1/Th2 in Serum of Mice Model of Damp-heat Syndrome of Seasonal Febrile Disease Infected by MHV-A59

Objectives:To study pathogenic mechanism of damp-heat syndrome of seasonal febrile disease from level of Th1/Th2 in serum of mice models of damp-heat syndrome of seasonal febrile disease infected by MHV-A59 and Bacillus coli.Methods:①Pathogenic mechanism of damp-heat syndrome of seasonal febrile disease:four-week-old mice of BALB/c were divided into three groups randomly,control group,model group,Bacillus coli group.The levels of IL-4 and IFN-γ in mice serum was detected by ELISA.②Analyze the position of etiological factor in damp-heat syndrome of seasonal febrile disease:four-week-old mice of BALB/c were divided into four groups randomly,control group,model group,Bacillus coli group,damp-heat group(rich fatty and sweet diet plus humid heat environment),MHV-A59 group(infecting by MHV-A59).The levels of IL-4 and IFN-γ in mice serum were detected by ELISA.③Certificat the pathogenic mechanism of damp-heat syndrome of seasonal febrile disease induced by MHV-A59 through treating this disease with the decoction of clearing away heat and dampness:four-week-old mice of BALB/c were divided into five groups randomly,control group,model group,A decoction group(mice of model group were treated by Haoqin Qingdan Decoction),B decoction group(mice of model group were treated by Coptidis Decoction for Detoxification),C decoction group(mice of model group were treated by Sanren Decoction).The levels of IL-4 and IFN-γ in mice serum were detected by ELISA.Results:①The Th1/Th2(IFN-γ/IL-4)of model group and Bacillus coli group were obviously increased compared to control group(P<0.05 or P<0.01).②The balance of Th1/Th2 and CD4+/CD8+ of model group became obviously disequilibrium compared to control group(P<0.05)because of MHV-A59 infecting.③ The disequilibrium of Th1/Th2 was adjusted obviously compared to model group(P<0.05).Conclusion:The disequilibrium of Th1/Th2 in serum was one of the mutual pathogenic mechanisms of damp-heat syndrome of seasonal febrile disease.MHV-A59 plays a key role disorder of T cell immunity of damp-heat syndrome of seasonal febrile disease,the decoction of clearing away heat and dampness could treat the disequilibrium of Th1/Th2.It is the evidence for the pathogenic mechanism referred in our study.